bpsbioscience，50223，BCL-XLTR-FRETAssayKit

Description:

The BCL-XL TR-FRET Assay Kit is designed to measure the inhibition of BCLXL binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL-XL, peptide ligand, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Synonym(s):

Bcl-Xlong, B cell lymphoma XL, HTRF, BCLXL, BCL2, BCL-2

Format:

COMPONENTS:

Instructions for use:

See assay kit data sheet for detailed protocol

Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Reference(s):

Lessene, G., et al., Nature Chem. Biol. 2013 ; 9:390

Notes:

Since January of 2018, this kit has been optimized for better performance. The previous version of the BCL-XL TR-FRET Assay Kit, Cat. #50223, can still be purchased upon special request.

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

Apoptosis

Product Type:

Assay Kit

bpsbioscience，50223，BCL…