bpsbioscience，79601，BCL2A1TR-FRETAssayKit

Description:

The BCL2A1 TR-FRET Assay Kit is designed to measure the inhibition of BCL2A1 (BFL1) binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL2A1, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Materials Required But Not Provided:

Dithiothreitol (DTT, 0.5 M) Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips

UniProt

Q16548

Synonym(s):

BCL2L5, BCL2-L5, BFL1, GRS, HBPA1, HTRF

Format:

COMPONENTS:

Instructions for use:

See assay kit data sheet for detailed protocol.

Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Reference(s):

Kvansakul, M., Hinds, MG. Cell Death and Disease 2013; 4: e909.

Warning(s):

Avoid freeze/thaw cycles

Scientific Category:

Apoptosis

Product Type:

Assay Kit

bpsbioscience，79601，BCL…