bpsbioscience，80700，Caspase-3HomogeneousAssayKit

Background:

Caspase-3 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.

Description:

The Homogeneous Caspase-3 Assay Kit is a complete assay system designed to measure Caspase-3 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-3 activity measurements. In addition, the kit includes purified Caspase-3 enzyme and a potent Caspase-3 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-3 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-3 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Synonym(s):

CASP3, caspase 3, CPP32, apopain, Yama, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen

Contraindications:

DMSO >1%, strong acids or bases, ionic detergents, high salt

Format:

Instructions for use:

See assay kit data sheet for detailed protocol.

Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Great for studying enzyme kinetics and HTS applications.

Reference(s):

1. Liu, J., et al., Bioorg. Med. Chem. Lett., 1999 Nov 15;9(22):3231-6.
2. Riedl, S.J., et al., Cell, 2001 Mar 9;104(5):791-800

Notes:

AlphaScreen^? and AlphaLISA^? are registered trademarks of PerkinElmer, Inc.

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

Protease

Product Type:

Assay Kit

bpsbioscience，80700，Cas…