bpsbioscience，79588，CathepsinSInhibitorScreeningAssayKit

Background:

Cathepsin S is a cysteine protease of the papain family, found inside the lysosomal/endosomal compartments of antigen-presenting cells such as B cells, macrophages and dendritic cells. Cathepsin S participates in the degradation of antigenic proteins to peptides for presentation to the MHC class II. Cathepsin S has additional biological effects, acting as elastase on the surface of vascular endothelial cells, a process that contributes to microvascular complications in diabetes mellitus.

Description:

The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well format, with purified Cathepsin S, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions. In addition, the kit includes the cathepsin inhibitor E64 for use as a control inhibitor.

Materials Required But Not Provided:

? 0.5 M DTT in aqueous solution
? Adjustable micropipettor and sterile tips
? Fluorescent microplate reader

Synonym(s):

Cathepsin S, CTSS, activity assay kit

Supplied As:

The Cathepsin S assay kit comes in a convenient 96-well format, with purified Cathepsin S, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.

Format:

COMPONENTS:

Instructions for use:

See assay kit data sheet for detailed protocol.

Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Reference(s):

Tato, M., et al. (2017). Cathepsin S inhibition combines control of systemic and peripheral pathomechanisms of autoimmune tissue injury. Scientific reports 7(1), 2775

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

Protease

Product Type:

Assay Kit

bpsbioscience，79588，Cat…