bpsbioscience，50272，BCL-2,His-tag

Species: Human

Host Species: E. coli

MW: 27 kDa

Genbank #: P10415

Tag(s): C-terminal His-tag

a.a: 1–211

Background:

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Description:

Human Bcl-2 (GenBank Accession No. P10415), a.a. 1-211 with C-terminal His-tag, MW = 27 kDa, expressed in an E. coli expression system.

UniProt

P10415

Synonym(s):

Bcl, Bcl2, B cell lymphoma 2

Assay Conditions:

Following the protocol for BPS Cat. #50222. Briefly, BCL-2 was combined with BCL-2 peptide ligand in TR-FRET assay buffer along with anti-His Tb donor and dyelabeled acceptor. Varying concentrations of ABT 199 were added and the reaction was incubated at room temperature for 2 hours followed by fluorescent intensity measurements.

Formulation:

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Format:

Aqueous buffer solution

Application(s):

Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.

Reference(s):

1. Labi V. et al., Cell Death Differ. 2008 Jun;15(6):977-87.
2. Rong Y.P. et al., Mol Cell. 2008 Jul 25;31(2):255-65.

Warning(s):

Avoid freeze/thaw cycles

Scientific Category:

Apoptosis

Product Type:

Recombinant Protein

目录＃	尺寸	产品名称
50272	100微克	BCL-2，His-tag
50273	100微克	BCL-XL，His-tag
100077	100微克	BCL2A1，His-Tag
100080	100微克	BCL2L10，His-Tag
100091	100微克	BCL2L2，His-Tag
80500	10微克 50微克	Caspase3，His-tag
80113	10微克 50微克	Caspase6，His-tag
70000	10微克 50微克	Caspase7，His-tag
80114	10微克 50微克	Caspase8，His-tag
80115	50微克	Caspase9，His-tag
40742	100微克	MCL1，His-Tag
80117	5微克	NLRP3（NALP3），His-FLAG标签（Sf9衍生）
100069	100微克	SARM1，FLAG-Tag
75003	20微克	STAT3，GST-tag
80016	50微克	WDR5，His-Tag，Biotin-Labeled
75002	50微克	XIAP（Bir2，Bir3），His-tag
75001	50微克	XIAP（Bir3），His-tag
80401	20微克	XIAP，FLAG-tag

bpsbioscience，50272，BCL…