爱尔兰Megazyme 膳食纤维总量检测试剂盒 Total Dietary Fibre Assay Kit K-TDFR-200A
货号:K-TDFR
品名:总膳食纤维检测试剂盒 Total Dietary Fibre Assay Kit
型号:200 Assays
膳食纤维是指碳水化合物及其相类似物质的总和,包括多糖、寡糖、木质素以及相关的植物物质。主要有纤维素、半纤维素、果胶及亲水胶体物质,如树胶、海藻多糖等组分;另外还包括植物细胞壁中所含有的木质素;不被人
体消HUA酶所分解的物质,如抗性淀粉、抗性糊精、抗性低聚糖、改性纤维素、黏质、寡糖以及少量相关成分,如蜡纸、角质、软木脂等。
总检测时间:100min
检测限:样品重量的0. 5-l00%
适用样品:配料成分、食品以及其他原料
优点:
价格低廉(每次检测成本)
所有试剂的稳定性>2年
采用高纯度/标准化的酶
操作简单
AOAC(Methods 985. 29, 991. 42, 991. 43和993. 19)和AACC (methods 32-05, 32-07和32-21)
参考文献:
1. Association of Official Analytical Chemists (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43,A20, p.399.
2. Association of Official Analytical Chemists (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69:370.
3. Association of Official Analytical Chemists (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70:393.
4. Prosky, L., Asp, N.-G., Furda, I., DeVries, J.W., Schweizer, T.F.and Harland, B.F. (1985).Determination of total dietary fibre in foods and food products: Collaborative study. J. Assoc.
The Total Dietary Fiber test kit is suitable is suitable for the measurement and analysis of Total Dietary Fiber.
For the determination of Total Dietary Fiber in cereal products,
foodstuffs, feeds and other materials
Principle:
(α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose
(protease)
(2) Protein + H2O → peptides
(3) Dietary fiber determined gravimetrically following alcohol
precipitation
(4) Ash and residual protein determined on DF residues
and subtracted
Kit size: 100 / 200 assays
Method: Hydrolysis / removal of non-dietary
fibre components
Total assay time: ~ 100 min
Detection limit: 0.5-100% of sample weight
Application examples:
Food ingredients, food products and other materials
Method recognition:
AOAC (Methods 985.29, 991.42, 991.43 and 993.19), AACC
(Methods 32-05.01, 32-06.01, 32-07.01 and 32-21.01) and
CODEX (Type I Method)
Total Dietary Fiber Assay Kit, for the measurement and analysis of total, soluble and insoluble dietary fiber according to AOAC and AACC approved methods. See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.
Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our booklet are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.
1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.
2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.
3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.
Two separate methods are described in the associated data booklet:
METHOD 1:
DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER
Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).
METHOD 2:
DETERMINATION OF TOTAL DIETARY FIBER
Based on AACC method 32-05.01 and AOAC Method 985.29.
Advantages
Very competitive price (cost per test)
All reagents stable for > 2 years
High purity / standardised enzymes employed
Mega-Calc? software tool is available from our website for hassle-free raw data processing
Simple format
中国食品安全国家标准GB 5009.88-2014.3966-食品中的膳食纤维的测定
爱尔兰Megazyme 膳食纤维总量检测试剂盒 Total Dietary Fibre Assay Kit K-TDFR-200A
爱尔兰Megazyme 膳食纤维总量检测试剂盒 Total Dietary Fibre Assay Kit K-TDFR-200A
爱尔兰Megazyme 膳食纤维总量检测试剂盒 Total Dietary Fibre Assay Kit K-TDFR-200A
爱尔兰Megazyme 膳食纤维总量检测试剂盒 Total Dietary Fibre Assay Kit K-TDFR-200A