内切纤维素酶检测试剂盒(CELLG3方法)
英文名：Cellulase Assay Kit (CELLG3 Method)
货号：MK-CELLG3
规格：180 / 360 assays per kit / 720 (auto-analyser)
For the specific measurement of cellulase (endo-1,4-β-glucanase) in enzyme preparations and fermentation products.
Colourimetric method for the determination of endo-1,4-β-
glucanase (cellulase) in enzyme preparations and fermentation
products
Principle:
(endo-1,4-β-glucanase)
(1) Benzylidene-G3-β-CNP + H2O → Blocked-GX + G(3-X)-β-CNP
(thermostable β-glucosidase)
(2) G(3-X)-β-CNP + H2O → D-glucose + CNP
(alkaline solution)
(3) CNP → phenolate ion (yellow colour)
Note: CNP = 2-Chloro-4-nitrophenol
Kit size: 180 / 360 assays / 720 (auto-analyser)
Method: Spectrophotometric at 400 nm
Total assay time: ~ 20 min
Detection lim-it: 0.05 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition: Novel method
Note: The analogous fluorimetric reagent (R-CELLF)
is also available with 10-fold greater sensitivity
Advantages
Very cost effective
All reagents stable for > 2 years after preparation
Completely specific for cellulase (endo-1,4-glucanase). The substrate is not hydrolysed by β-glucosidase, cellbiohyrolase or any other enzymes tested
Kinetic assays possible due to significant phenolate ion presence (and UV absorbance) at pH 5-6
Simple format. Well suited to automation
Standard included
爱尔兰Megazyme 内切纤维素酶检测试剂盒(CELLG3方法) Cellulase Assay Kit (CELLG3 Method) K-CELLG3
爱尔兰Megazyme 内切纤维素酶检测试剂盒(CELLG3方法) Cellulase Assay Kit (CELLG3 Method) K-CELLG3
爱尔兰Megazyme 内切纤维素酶检测试剂盒(CELLG3方法) Cellulase Assay Kit (CELLG3 Method) K-CELLG3
爱尔兰Megazyme 内切纤维素酶检测试剂盒(CELLG3方法) Cellulase Assay Kit (CELLG3 Method) K-CELLG3
爱尔兰Megazyme 内切纤维素酶检测试剂盒(CELLG3方法) Cellulase Assay Kit (CELLG3 Method) K-CELLG3