V01077必拓生物pTFN载体，pTFN vector，质粒载体图谱
pTFN载体含有分子伴侣FkpA编码序列。大肠杆菌分子伴侣FkpA同时具有肽酰脯氨酰顺反异构酶活性和分子伴侣活性，可以促进外源蛋白的正确折叠，有利于外源蛋白的表达。除此之外，pTFN载体还含有T7启动子，可用于外源蛋白在大肠杆菌体系中的高效表达。载体大小约为6.1kbp，卡钠霉素抗性。
产品特色
1.含有分子伴侣FkpA，可促进外源蛋白正确折叠与表达；
2.含有强启动子T7启动子，表达效率较高
成分
10 mM Tris（pH8.0）
保存条件
-20℃保存
注意事项
1. T7启动子强度较高，为提高可溶表达量，可以适当减少诱导剂的浓度或者降低诱导时的温度；
2.目的蛋白以融合的方式表达在FkpA下游，载体C-末端同时含有His6标签序列，可用于纯化。
产品目录
| 货号 | 产品名称 | 规格 | 报价(元) |
| V01077 | pTFN vector | 10μl，20ng/μl | 2000 |
必拓生物pTFN载体，pTFN vector，质粒载体图谱
LOCUS pTFN 6091 bp DNA circular 17-NOV-2016
SOURCE
ORGANISM
FEATURES Location/Qualifiers
promoter 21..37
/vntifkey="30"
/label=T7\promoter
misc_feature complement(1691..2503)
/vntifkey="21"
/label=kan
misc_feature complement(4646..5725)
/vntifkey="21"
/label=lacI
misc_feature 972..989
/vntifkey="21"
/label=His6
misc_feature 108..917
/vntifkey="21"
/label=E.coli\FkpA
rep_origin 2549..3682
/vntifkey="33"
/label=ColE1\Origin
BASE COUNT 1496 a 1596 c 1573 g 1426 t
ORIGIN
1 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
61 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg aaatcactgt
121 ttaaagtaac gctgctggcg accacaatgg ccgttgccct tcatgcacca atcacttttg
181 ctgctgaagc tgcaaaacct gctacagctg ctgacagcaa agcagcgttc aaaaatgacg
241 atcagaaatc agcttatgca ctgggtgcct cgctgggtcg ttacatggaa aactctctaa
301 aagaacaaga aaaactgggc atcaaactgg ataaagatca gctgattgct ggtgttcagg
361 atgcatttgc tgataagagc aaactctccg accaagagat cgaacagact ctacaagcat
421 tcgaagctcg cgtgaagtct tctgctcagg cgaagatgga aaaagacgcg gctgataacg
481 aagcaaaagg taaagagtac cgcgagaaat ttgccaaaga gaaaggtgtg aaaacctctt
541 caactggtct ggtttatcag gtagtagaag ccggtaaagg cgaagcaccg aaagacagcg
601 atactgttgt agtgaactac aaaggtacgc tgatcgacgg taaagagttc gacaactctt
661 acacccgtgg tgaaccgctt tctttccgtc tggacggtgt tatcccgggt tggacagaag
721 gtctgaagaa catcaagaaa ggcggtaaga tcaaactggt tattccacca gaactggctt
781 acggcaaagc gggtgttccg gggattccac cgaactctac cctggtgttt gacgtagagc
841 tgctggatgt gaaaccagcg ccgaaggctg atgcaaagcc ggaagctgat gcgaaagccg
901 cagattctgc taaaaaagag ctcatgtacc cgcgcggtaa cctcgagcat atggaattcg
961 gatccgtcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
1021 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
1081 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
1141 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
1201 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
1261 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
1321 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
1381 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
1441 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
1501 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
1561 cgcgaatttt aacaaaatat taacgtttac aatttcaggt ggcacttttc ggggaaatgt
1621 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa
1681 ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
1741 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
1801 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
1861 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
1921 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
1981 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
2041 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
2101 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
2161 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
2221 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
2281 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
2341 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
2401 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
2461 atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
2521 tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg
2581 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
2641 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
2701 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
2761 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
2821 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
2881 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
2941 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
3001 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
3061 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
3121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
3181 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
3241 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
3301 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
3361 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
3421 acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg
3481 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
3541 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
3601 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
3661 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc
3721 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
3781 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
3841 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
3901 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
3961 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg
4021 cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat
4081 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
4141 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
4201 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
4261 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggcgat
4321 aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag
4381 ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa
4441 gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat
4501 aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct
4561 gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta
4621 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
4681 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
4741 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
4801 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
4861 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
4921 agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
4981 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
5041 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
5101 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
5161 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
5221 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
5281 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
5341 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
5401 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
5461 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
5521 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
5581 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
5641 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
5701 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct
5761 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct
5821 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
5881 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
5941 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
6001 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
6061 ccggccacga tgcgtccggc gtagaggatc g
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