V01061必拓生物pTWIN1载体，pTWIN1 vector，质粒载体图谱
含有蛋白内含子（Intein）序列，可在蛋白水平自剪切
成分
10 mM Tris（pH8.0）
保存条件
-20℃保存
产品目录
| 货号 | 产品名称 | 规格 | 报价(元) |
| V01061 | pTWIN1 vector | 10μl，20ng/μl | 800 |
必拓生物pTWIN1载体，pTWIN1 vector，质粒载体图谱
LOCUS pTWIN1 7375 bp DNA circular SYN 2-MAY-2017
SOURCE synthetic DNA construct.
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
source 1..7375
/organism="synthetic DNA construct"
/mol_type="other DNA"
/vntifkey="98"
CDS 140..1000
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/vntifkey="4"
/label=bla（AmpR）
/note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
CDS complement(2623..2814)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/vntifkey="4"
/label=rop
/note="rop"
CDS complement(3371..4453)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/vntifkey="4"
/label=lacI
/note="lacI The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 5637..5655
/vntifkey="30"
/label=Promoter_P_1
/note="T7 promoter promoter for bacteriophage T7 RNA polymerase"
RBS 5695..5717
/vntifkey="32"
/label=RBS_1
/note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
CDS 5749..5901
/codon_start=1
/gene="Bacillus circulans chiA"
/product="chitin binding domain from chitinase A1 (Watanabe
et al., 1994)"
/vntifkey="4"
/label=Bacillus\circulans\chiA
/note="CBD"
CDS 6445..7038
/codon_start=1
/gene="Mycobacterium xenopi gyrA"
/product="modified GyrA intein (Southworth et al., 1999)"
/vntifkey="4"
/label=Mycobacterium\xenopi\gyrA
/note="Mxe GyrA intein N-terminal cleavage of this intein can be induced by thiols."
CDS 7069..7224
/codon_start=1
/gene="Bacillus circulans chiA"
/product="chitin binding domain from chitinase A1 (Watanabe
et al., 1994)"
/vntifkey="4"
/label=Bacillus\circulans\chiA
/note="CBD"
BASE COUNT 1828 a 1884 c 1985 g 1678 t
ORIGIN
1 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
301 ccttgagagt tttcgccccg aagaacgttc tccaatgatg agcactttta aagttctgct
361 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
1021 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag
1081 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
1141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
1201 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
1261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
1321 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
1381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
1441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
1501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag
1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
2341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
2401 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg
2461 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
2521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
2581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
2641 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc
2701 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
2761 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
2821 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
2881 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
2941 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc
3001 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
3061 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
3121 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
3181 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
3241 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
3301 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
3361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
3421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
3481 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
3541 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
3601 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
3661 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
3721 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
3781 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
3841 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
3901 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
3961 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
4021 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
4081 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
4141 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
4201 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
4261 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
4321 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
4381 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
4441 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
4501 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac
4561 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
4621 aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca attccccagg
4681 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
4741 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
4801 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc
4861 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
4921 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
4981 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc
5041 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
5101 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
5161 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa
5221 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
5281 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
5341 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat
5401 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
5461 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
5521 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
5581 aattggggat cggaattaat tcccggttta aaccggggat ctcgatcccg cgaaattaat
5641 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
5701 taactttaag aaggagatat acatatgaaa atcgaagaag gtaaactgac aaatcctggt
5761 gtatccgctt ggcaggtcaa cacagcttat actgcgggac aattggtcac atataacggc
5821 aagacgtata aatgtttgca gccccacacc tccttggcag gatgggaacc atccaacgtt
5881 cctgccttgt ggcagcttca aaacaacggt aacaacggtc tcgaactgcg cgagtccgga
5941 gctatctctg gcgatagtct gatcagcctg gctagcacag gaaaaagagt ttctattaaa
6001 gatttgttag atgaaaaaga ttttgaaata tgggcaatta atgaacagac gatgaagcta
6061 gaatcagcta aagttagtcg tgtattttgt actggcaaaa agctagttta tattctaaaa
6121 actcgactag gtagaactat caaggcaaca gcaaatcata gatttttaac tattgatggt
6181 tggaaaagat tagatgagct atctttaaaa gagcatattg ctctaccccg taaactagaa
6241 agctcctctt tacaattgtc accagaaata gaaaagttgt ctcagagtga tatttactgg
6301 gactccatcg tttctattac ggagactgga gtcgaagagg tttttgattt gactgtgcca
6361 ggaccacata actttgtcgc gaatgacatc attgtacaca acggaagagc catgggcggc
6421 cgcgaattcc tcgagggctc ttcctgcatc acgggagatg cactagttgc cctacccgag
6481 ggcgagtcgg tacgcatcgc cgacatcgtg ccgggtgcgc ggcccaacag tgacaacgcc
6541 atcgacctga aagtccttga ccggcatggc aatcccgtgc tcgccgaccg gctgttccac
6601 tccggcgagc atccggtgta cacggtgcgt acggtcgaag gtctgcgtgt gacgggcacc
6661 gcgaaccacc cgttgttgtg tttggtcgac gtcgccgggg tgccgaccct gctgtggaag
6721 ctgatcgacg aaatcaagcc gggcgattac gcggtgattc aacgcagcgc attcagcgtc
6781 gactgtgcag gttttgcccg cggaaaaccc gaatttgcgc ccacaaccta cacagtcggc
6841 gtccctggac tggtgcgttt cttggaagca caccaccgag acccggacgc ccaagctatc
6901 gccgacgagc tgaccgacgg gcggttctac tacgcgaaag tcgccagtgt caccgacgcc
6961 ggcgtgcagc cggtgtatag ccttcgtgtc gacacggcag accacgcgtt tatcacgaac
7021 gggttcgtca gccacgctac tggcctcacc ggtctgaact caggcctcac gacaaatcct
7081 ggtgtatccg cttggcaggt caacacagct tatactgcgg gacaattggt cacatataac
7141 ggcaagacgt ataaatgttt gcagccccac acctccttgg caggatggga accatccaac
7201 gttcctgcct tgtggcagct tcaatgactg caggaagggg atccggctgc taacaaagcc
7261 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
7321 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggat
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