V010018必拓生物pCLDC载体，pCLDC vector，质粒载体图谱
成分：10 mM Tris（pH8.0）
保存条件：-20℃保存
产品目录
| 货号 | 产品名称 | 规格 | 报价(元) |
| V01018 | pCLDC vector | 10μl，20ng/μl | 1000
|
pCLDC载体，pCLDC vector，质粒载体图谱
LOCUS pCLDC 4377 bp DNA circular SYN 17-NOV-2016
FEATURES Location/Qualifiers
source 1..4377
/organism="Expression vector pColdIII"
/mol_type="other DNA"
/db_xref="taxon:289231"
/lab_host="Escherichia coli"
/vntifkey="98"
/note="expresses an open reading frame as a fusion to the translation enhancing element. This plasmid is derived from pUC118."
gene 15..482
/gene="cspA"
/vntifkey="60"
promoter 15..81
/gene="cspA"
/vntifkey="30"
/label=cspA\promoter
enhancer 256..270
/gene="cspA"
/vntifkey="9"
/label=cspA\TEE
/note="TEE (translation enhancing element)"
gene 1341..2201
/gene="Amp"
/vntifkey="60"
CDS 1341..2201
/gene="Amp"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="BAD35138.1"
/db_xref="GI:51090254"
/vntifkey="4"
/label=Amp
rep_origin 2361..2975
/vntifkey="33"
/label=ColE1\Origin
/note="ColE1 origin"
gene complement(3132..4214)
/gene="lacI"
/vntifkey="60"
CDS complement(3132..4214)
/gene="lacI"
/codon_start=1
/transl_table=11
/product="lactose operon repressor"
/protein_id="BAD35139.1"
/db_xref="GI:51090255"
/vntifkey="4"
/label=lacI
BASE COUNT 1102 a 1079 c 1139 g 1057 t
ORIGIN
1 aaggaatggt gtggccgatt aatcataaat atgaaaaata attgttgcat cacccgccaa
61 tgcgtggctt aatgcacatc aaattgtgag cggataacaa tttgatgtgc tagcgcatat
121 ccagtgtagt aaggcaagtc ccttcaagag ttatcgttga tacccctcgt agtgcacatt
181 cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc gaaaggcaca cttaattatt
241 aagaggtaat acaccatgaa tcacaaagtg catatggagc tcggtaccct cgagggatcc
301 gaattcaagc ttgtcgacct gcagtctaga taggtaatct ctgcttaaaa gcacagaatc
361 taagatccct gccatttggc ggggattttt ttatttgttt tcaggaaata aataatcgat
421 cgcgtaataa aatctattat tatttttgtg aagaataaat ttgggtgcaa tgagaatgcg
481 caggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
541 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
601 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
661 tgtactgaga gtgcaccata aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
721 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
781 atcaaaagaa tagcccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
841 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
901 actacgtgaa ccatcaccca aatcaagttt tttggggtcg aggtgccgta aagcactaaa
961 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
1021 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
1081 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtacta
1141 tggttgcttt gacgtatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
1201 atcaggcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
1261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
1321 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
1381 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
1441 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
1501 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
1561 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
1621 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
1681 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
1741 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
1801 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
1861 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
1921 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
1981 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
2041 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
2101 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
2161 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
2221 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
2281 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
2341 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
2401 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
2461 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
2521 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
2581 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
2641 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
2701 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
2761 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
2821 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
2881 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
2941 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
3001 gctggccttt tgctcacata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
3061 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa
3121 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
3181 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
3241 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
3301 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
3361 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
3421 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
3481 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
3541 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
3601 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
3661 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
3721 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
3781 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
3841 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
3901 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
3961 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
4021 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
4081 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
4141 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
4201 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
4261 ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga
4321 ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgc
//