V01015必拓生物pTac28载体，pTac28 vector，质粒载体图谱
成分：10 mM Tris（pH8.0）
保存条件：-20℃保存
产品目录
| 货号 | 产品名称 | 规格 | 报价(元) |
| V01015 | pTac28 vector | 10μl，20ng/μl | 800 |
pTac28载体，pTac28 vector，质粒载体图谱
LOCUS pTac28 5414 bp DNA circular 17-NOV-2016
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
FEATURES Location/Qualifiers
misc_feature 295..312
/vntifkey="21"
/label=His6
misc_feature complement(1014..1826)
/vntifkey="21"
/label=kan
misc_feature complement(3969..5048)
/vntifkey="21"
/label=lacI
promoter 33..61
/vntifkey="30"
/label=tac\promoter
misc_feature 165..182
/vntifkey="21"
/label=His6
rep_origin 1872..3005
/vntifkey="33"
/label=ColE1\Origin
BASE COUNT 1276 a 1456 c 1404 g 1278 t
ORIGIN
1 agatctctgg caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg
61 tgtggaattg tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt
121 tcacgagcac ttcaccaaca aggaccatag ccatgggcag cagccatcat catcatcatc
181 acagcagcgg cctggtgccg cgcggcagcc atatggctag catgactggt ggacagcaaa
241 tgggtcgcgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac
301 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
361 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
421 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
481 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
541 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
601 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
661 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
721 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
781 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
841 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
901 tattaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
961 gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac
1021 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
1081 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
1141 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
1201 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
1261 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
1321 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
1381 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
1441 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
1501 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
1561 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
1621 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
1681 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
1741 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa
1801 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
1861 agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
2281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
2341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
2461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
2521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
2581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
2641 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
2701 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
2761 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
2821 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
2881 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
2941 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
3001 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
3061 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
3121 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
3181 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
3241 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
3301 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
3361 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
3421 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg
3481 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
3541 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
3601 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc
3661 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga
3721 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa
3781 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa
3841 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
3901 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
3961 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
4021 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
4081 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
4141 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
4201 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg
4261 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
4321 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
4381 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
4441 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
4501 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
4561 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
4621 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
4681 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
4741 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
4801 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
4861 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
4921 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
4981 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
5041 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
5101 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
5161 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
5221 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
5281 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
5341 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
5401 ggcgtagagg atcg
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