V01004必拓生物pET32a(+)载体，pET32a(+) vector，质粒载体图
N-末端TrxA融合标签
成分：10 mM Tris（pH8.0）
保存条件：-20℃保存
产品目录
| 货号 | 产品名称 | 规格 | 报价(元) |
| V01004 | pET32a(+) vector | 10μl，20ng/μl | 700 |
pET32a(+)载体，pET32a(+) vector，质粒载体图
LOCUS pET-32a(+) 5896 bp DNA circular 15-NOV-2017
SOURCE
ORGANISM
FEATURES Location/Qualifiers
misc_feature 1399..2256
/vntifkey="21"
/label=bla(Amp)
promoter 21..37
/vntifkey="30"
/label=T7\promoter
misc_feature 644..661
/vntifkey="21"
/label=His6
misc_feature complement(4451..5530)
/vntifkey="21"
/label=lacI
rep_origin 2320..3487
/vntifkey="33"
/label=ColE1\origin
misc_feature 109..435
/vntifkey="21"
/label=E.coli\thioredoxin(trxA)
BASE COUNT 1385 a 1559 c 1562 g 1390 t
ORIGIN
1 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
61 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gagcgataaa
121 attattcacc tgactgacga cagttttgac acggatgtac tcaaagcgga cggggcgatc
181 ctcgtcgatt tctgggcaga gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat
241 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcaaaac
301 cctggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac
361 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc
421 gacgctaacc tggccggttc tggttctggc catatgcacc atcatcatca tcattcttct
481 ggtctggtgc cacgcggttc tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag
541 cacatggaca gcccagatct gggtaccgac gacgacgaca aggccatggc tgatatcgga
601 tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
661 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
721 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
781 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
841 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
901 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
961 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
1021 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
1081 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
1141 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
1201 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
1261 acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
1321 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
1381 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
1441 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
1501 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
1561 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
1621 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
1681 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
1741 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
1801 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
1861 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
1921 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
1981 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
2041 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
2101 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
2161 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
2221 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
2281 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
2341 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
2401 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
2461 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
2521 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
2581 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
2641 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
2701 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
2761 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
2821 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
2881 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
2941 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
3001 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
3061 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
3121 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
3181 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
3241 atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
3301 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
3421 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
3481 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
3541 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
3601 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
3661 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
3721 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
3781 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
3841 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
3901 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
3961 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
4021 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
4081 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
4141 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
4201 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
4261 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
4321 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
4381 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
4441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
4501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
4561 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
4621 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
4681 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
4741 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
4801 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
4861 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
4921 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
4981 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
5041 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
5101 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
5161 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
5221 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
5281 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
5341 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
5401 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
5461 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
5521 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
5581 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
5641 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
5701 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
5761 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
5821 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
5881 ccggcgtaga ggatcg
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