V01002 必拓生物pET28a(+)载体，pET28a(+) vector，质粒载体图谱
N-末端、C-末端His6双标签
成分:10 mM Tris（pH8.0）
保存条件:-20℃保存
产品目录
| 货号 | 产品名称 | 规格 | 报价(元) |
| V01002 | pET28a(+) vector | 10μl，20ng/μl | 500
|
pET28a(+)载体，pET28a(+) vector，质粒载体图谱
LOCUS pET28a(+) 5369 bp DNA circular 17-NOV-2016
SOURCE
ORGANISM
FEATURES Location/Qualifiers
misc_feature 250..267
/vntifkey="21"
/label=His6
promoter 21..37
/vntifkey="30"
/label=T7\promoter
misc_feature complement(969..1781)
/vntifkey="21"
/label=kan
misc_feature complement(3924..5003)
/vntifkey="21"
/label=lacI
misc_feature 120..137
/vntifkey="21"
/label=His6
misc_feature 147..164
/vntifkey="21"
/label=Thrombin\site
rep_origin 1827..2960
/vntifkey="33"
/label=ColE1\Origin
BASE COUNT 1266 a 1444 c 1391 g 1268 t
ORIGIN
1 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
61 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
121 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg gctagcatga
181 ctggtggaca gcaaatgggt cgcggatccg aattcgagct ccgtcgacaa gcttgcggcc
241 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
301 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
361 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
421 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
481 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
541 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
601 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
661 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
721 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
781 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
841 cgaattttaa caaaatatta acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc
901 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt
961 aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
1021 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
1081 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
1141 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
1201 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
1261 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
1321 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
1381 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
1441 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
1501 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
1561 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
1621 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
1681 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
1741 cgcggcctag agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
1801 tttatgtaag cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt
1861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
1921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
1981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
2041 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
2101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
2161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
2221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
2281 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
2341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
2401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
2461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
2521 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
2581 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
2641 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
2701 gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat
2761 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
2821 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
2881 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
2941 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
3001 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
3061 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
3121 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
3181 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
3241 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
3301 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
3361 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
3421 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
3481 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
3541 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa
3601 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
3661 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
3721 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
3781 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
3841 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
3901 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
3961 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
4021 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
4081 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg
4141 gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag
4201 atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc
4261 tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt
4321 tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga
4381 ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat
4441 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
4501 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca
4561 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
4621 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct
4681 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
4741 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
4801 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
4861 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
4921 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
4981 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
5041 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc
5101 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
5161 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
5221 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
5281 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
5341 ggccacgatg cgtccggcgt agaggatcg
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