The D-3-Hydroxybutyric Acid Assay Kit is suitable for the specific measurement and analysis of D-hydroxybutyric acid in eggs and egg products and other foods and beverages. Suitable for manual, auto-analyser and microplate formats.
For the rapid and specific assay of D-lactic acid in wine, beer, juice, milk, cheese, vinegar and meat products. Content:50 assays per kit
Advantages
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Very rapid reaction (~ 3 min)
No wasted diaphorase solution (stable suspension supplied)
Mega-Calc? software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Colourimetric method for the determination of D-3-Hydroxybutyric Acid in foodstuffs
Principle:
(3-hydroxybutyrate dehydrogenase)
(1) D-3-Hydroxybutyrate + NAD+ ? acetoacetate + NADH + H+
(diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan
Kit size: 60 assays (manual) / 600 (microplate)
/ 740 (auto-analyser)
Method: Spectrophotometric at 492 nm
Reaction time: ~ 3 min
Detection limit: 0.20 mg/L
Application examples:
Egg, egg products (e.g. egg powder) and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by CEC