The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.
UV-method for the determination of Glucomannan in plant
products, foodstuffs and other materials
Principle:
(β-mannanase)
(1) Ac-Glucomannan + H2O → Ac-glucomanno-oligomers
(pH 12.5)
(2) Ac-Glucomanno-oligomers + H2O → glucomanno-oligomers
+ acetate
(β-glucosidase + β-mannosidase)
(3) Glucomanno-oligomers + H2O → D-glucose + D-mannose
(hexokinase)
(4) D-glucose + D-mannose + ATP → G-6-P + M-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphomannose isomerase) (phosphoglucose isomerase)
(6) M-6-P → F-6-P → G-6-P
Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Total assay time: 120 min
Detection limit: 1-100% of sample weight
Application examples:
Jelly sweets, cosmetics, food gums and other materials
Method recognition: Novel method
Advantages
Very cost effective
Only enzymatic kit available
Simple format
All reagents stable for > 2 years after preparation
Mega-Calc? software tool is available from our website for hassle-free raw data processing
Standard included