The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of L-Glutamine and Ammonia
in cell culture media, foodstuffs and other materials
Principle:
(glutaminase)
(1) L-Glutamine + H2O → L-glutamate + NH4+
(microbial glutamate dehydrogenase)
(2) NH4+ + 2-Oxoglutarate + NADPH → L-glutamate + NADP+ + H2O
Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 0.54 mg/L (L-glutamine)
0.06 mg/L (ammonia)
Application examples:
Cell culture media and cultures, dietary supplements, vegetables
and other materials (e.g. biological samples, etc.)
Method recognition: Novel method
Advantages
Very rapid reaction due to use of high activity glutaminase and uninhibited glutamate dehydrogense
All enzymes supplied as stabilised suspensions
Only enzymatic kit available
Very cost effective
All reagents stable for > 2 years after preparation
Mega-Calc? software tool is available from our website for hassle-free raw data processing
Standard included