The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.
Glucose Oxidase assay kit is suitable for manual, auto-analyser and microplate formats.
Colourimetric method for the determination of Glucose Oxidase
in foodstuffs and fermentation products
Principle:
(glucose oxidase)
(1) D-Glucose + H2O + O2 → D-glucono-δ-lactone + H2O2
(peroxidase)
(2) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Kit size: 200 assays (manual) / 2000 (microplate)
/ 1960 (auto-analyser)
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 10 U/L
Application examples:
Enzyme preparations, and other materials (e.g. biological cultures,
samples, etc.)
Method recognition: Novel method
Advantages
Very competitive price (cost per test)
All reagents stable for > 12 months after preparation
Simple format
Mega-Calc? software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats