The D-/L-Lactic Acid (D-/L-Lactate) (Rapid) test kit is used for the rapid and specific concurrent measurement and analysis of L-lactic acid (L-lactate) and D-lactic acid (D-lactate) in beverages, meat, dairy and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of D-/L-Lactic Acid in
foodstuffs, beverages and other materials
Principle:
(D-lactate dehydrogenase)
(1) D-Lactic acid + NAD+ ? pyruvate + NADH + H+
(L-lactate dehydrogenase)
(2) L-Lactic acid + NAD+ ? pyruvate + NADH + H+
(glutamate-pyruvate transaminase)
(3) Pyruvate + D-glutamate → D-alanine + 2-oxoglutarate
Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 10 min (L-lactic acid) and ~ 5 min (D-lactic acid)
Detection limit: 0.21 mg/L
Application examples:
Wine, soft drinks, milk, dairy products, foods containing milk (e.g. dietetic
foods, bakery products, baby food, chocolate, sweets and ice-cream),
vinegar, fruit and vegetables, processed fruit and vegetables, meat
products, food additives, paper (and cardboard), cosmetics, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by DIN, GOST,
IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAK
Advantages
Rapid total analysis time (concurrent / flexible D and L-lactic acid reaction format)
D-lactate dehydrogenase reaction very rapid with most samples (~ 5 min)
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Mega-Calc? software tool is available from our website for hassle-free raw data processing
Standard included
Extended cofactors stability