The D-Fructose and D-Glucose MegaQuant? Format test kit is suitable for the measurement and analysis of D-fructose and D-glucose in grapes, grape juice and wine using the MegaQuant? colorimeter (measurement at 505 nm).
Simple colourimetric method for the determination of
D-Fructose and D-Glucose in foodstuffs, beverages and
other materials
Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP
(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP
(phosphoglucose isomerase)
(3) F-6-P ? G-6-P
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(diaphorase)
(5) INT + NADPH + H+ → NADP+ + INT-formazan
Kit size: 60 assays
Method: Spectrophotometric at 505 nm
Reaction time: ~ 10 min
Detection limit: 15.4 mg/L
Application examples:
Grape juice / must, wine, beer, fruit juices, soft drinks, milk, jam, honey,
dietetic foods, bread, bakery products, candies, desserts, confectionery,
ice-cream, fruit and vegetables, condiments, tobacco, cosmetics,
pharmaceuticals, paper and other materials (e.g. biological cultures,
samples, etc.)
Method recognition: Novel method
Advantages
Novel product, patented technology
Spectrophotometer / laboratory expertise not required
Highly stable reagents (at least three seasons use)
Very competitive price (cost per test)
Very simple procedure
Rapid reaction time (~ 10 min)
Standard included