The D-Fructose/D-Glucose (Liquid Ready Reagents) test kit is a rapid, reliable and accurate method for the specific measurement and analysis of D-fructose and D-glucose in wine, beverages, foodstuffs and other materials. Supplied as a "ready to use" liquid stable formulation that is suitable for auto-analyser and microplate formats.
Suitable for auto-analyser and microplate formats.
UV-method suitable for auto-analyser and microplate formats for
the determination of D-Fructose and D-Glucose in foodstuffs,
beverages and other materials
Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP
(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphoglucose isomerase)
(4) F-6-P ? G-6-P
Kit size: 1100 assays (microplate) / 1100 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 13 min
Detection limit: 133 mg/L (recommended format)
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods,
bread, bakery products, candies, desserts, confectionery, ice-cream,
fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals,
paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC
Advantages
PVP incorporated to prevent tannin inhibition
“Ready to use” liquid stable formulation
Very competitive price (cost per test)
All reagents stable for > 2 years
Very rapid reaction (~ 13 min)
Standard included
Suitable for microplate and auto-analyser formats