The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharide.
UV-method for the determination of Fructan in foodstuffs,
beverages and other materials
Principle:
(sucrase + maltase)
(1) Sucrose + maltosaccharides + H2O → D-glucose + D-fructose
(exo-inulinase + endo-inulinase)
(2) Fructan + H2O → D-glucose + D-fructose
(hexokinase)
(3) D-Glucose + D-fructose + ATP → G-6-P + F-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphoglucose isomerase)
(5) F-6-P ? G-6-P
Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Flours, plant materials (e.g. onion), food products and other materials
Method recognition:
This method is a modification of AOAC Method 999.03 and AACC
Method 32-32.01
Advantages
Very cost effective
All reagents stable for > 12 months after preparation
Fructan kits are available only from Megazyme
Simple format
Mega-Calc? software tool is available from our website for hassle-free raw data processing
Standard included