tmTNF抗体(跨膜型TNF-α的检测)-ab6671
产品名称:Anti-TNF alpha抗体
描述:兔多克隆抗体to TNF alpha
特异性:No reactivity is detected against human TNF-b (lymphotoxin). This antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin.
经测试应用: 适用于: ELISA, IHC-P, IHC-Fr, Neutralising, WB, ICC/IF, IHC-FoFr
种属反应性:与反应: Mouse, Rat, Guinea pig, Human, Pig, Fish, Cynomolgus Monkey
预测可用于: Dog, Monkey, Non Human Primates
免疫原:Recombinant full length protein corresponding to TNF alpha.
应用 | Ab评论 | 说明 |
---|
ELISA |
| 1/200 - 1/1000. |
IHC-P | | 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IHC-Fr | | 1/100 - 1/200. |
Neutralising |
| 1/200. |
WB | | 1/500 - 1/2000. Can be blocked with Recombinant Human TNF alpha protein (ab140754). Membrane Blocking is recommended with BSA not Milk for this product. Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. |
ICC/IF | | Use at an assay dependent concentration. PubMed: 19458984 |
IHC-FoFr |
| Use at an assay dependent concentration. |
tmTNF抗体(跨膜型TNF-α的检测)-ab6671
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