超高速激光拉曼显微成像系统

所属类别： ? 专用实验设备 ? 拉曼成像系统
           所属品牌：加拿大Photon etc公司

                                                                     产品简介

快速global mapping（非扫描式）

百万像素拉曼光谱，成像时间仅几分钟

斯托克斯和反斯托克斯

高光谱分辨率和空间分辨率

Photon etc公司RIMA拉曼成像技术是新一代快速、高精度、面扫描激光拉曼技术，它将共聚焦显微技术与激光拉曼光谱技术完美结合，与传统的点成像拉曼系统不同，采用面成像技术，将激光扩束后，用特殊的光学元件将扩束后的高斯分布的激光整形成均匀分布的平顶激光，照射在样品上，滤除反射的激光后，所有激发的拉曼光和再通过可调滤波器为主的高光谱成像组件，成像在ccd上，可在几分钟内完成，以相元为单位，可以形成高达十万组拉曼光谱数据。是目前市面上最快的拉曼成像设备。

关键字：拉曼成像系统，高速拉曼成像光谱仪，激光拉曼成像光谱仪，高速拉曼光谱成像仪，拉曼成像高光谱，激光拉曼高光谱成像系统，激光拉曼高光谱成像仪，RAMAN imaging system，RAMAN spectrometer，RAMAN spectral system

RIMA拉曼显微高光谱成像系统

Photon etc公司RIMA拉曼成像技术是新一代快速、高精度、面扫描激光拉曼技术，它将共聚焦显微技术与激光拉曼光谱技术完美结合，与传统的点成像拉曼系统不同，采用面成像技术，将激光扩束后，用特殊的光学元件将扩束后的高斯分布的激光整形成均匀分布的平顶激光，照射在样品上，滤除反射的激光后，所有激发的拉曼光和再通过可调滤波器为主的高光谱成像组件，成像在ccd上，可在几分钟内完成，以相元为单位，可以形成高达十万组拉曼光谱数据。是目前市面上最快的拉曼成像设备。

产品特点

1.      快速global mapping（非扫描式）

2.      百万像素拉曼光谱，成像时间仅几分钟

3.      斯托克斯和反斯托克斯

4.      高光谱分辨率和空间分辨率

设备原理图：

系统参数：

应用领域：

纳米材料分析

Global Raman imaging is an exceptional technique for the analysis oflarge surfaces of thin films and advanced materials. Its rapidity makes it agreat tool not only for universities and research institutes, but also forindustrial laboratories. With no or minimal sample preparation, RIMA?, Photon etc.’s newhyperspectral Raman imager, can easily take part in routine analysis, where theprompt access to information about sample composition is crucial for thedevelopment of new materials.

With systems based on point-to-point or scanning technologies, theacquisition of maps of large areas is often tedious and time consuming: theanalysis of a sample may take hours. RIMA? expedites in minutes theacquisition of the whole area in the field of view, rendering full maps of asample with unmatched rapidity. In fact, the hyperspectral cube is built imageby image, along the spectral window of interest, with a spectral resolutionbetter than 7 cm--1. Since a spectrum is recorded for each pixel, it ispossible, with a 1024 x 1024 pixels camera, to collect more than one millionspectra without moving the sample. Moreover, the size of the maps can be aslarge as 650 x 650 mm2, depending on the magnification of the objective usedfor the analysis. Photon etc.’s filters used for hyperspectral imaging arebased on holographic gratings, and provide very high efficiency for an optimalacquisition of the weak Raman scattering. Combined with top of the line lownoise CCD or EMCCD cameras, RIMA? is the most efficient Raman imagingsystem on the market.

In order to show the advantages of RIMA? in the analysis ofnanomaterials in biological systems, carbon nanotubes (CNT) have been incubatedwith a sample of Candida Albicans yeast cells and exposed to ahomogeneous (flat-top) laser excitation of 532 nm on the entire field of view.With a 50X objective, an area of 260 x 130 μm2 was imaged, with a step of4.5 cm--1 and an exposition time of 15 s. The complete analysis took 20minutes, for a total of more than 60,000 spectra.

Figure 1 shows the Raman hyperspectral cube of a portion of the imagedarea containing the yeast. The monochromatic Raman images revealed the positionof the aggregated yeast cells stained with the CNTs. The typical signal of CNTs(red line) confirmed their presence on the yeast cells, while in other areasthe hyperspectral camera did not detect any CNT Raman signal (blue line).

Raman Multiplexing

DEVELOPMENTAND CHARACTERIZATION OF CARBON NANOTUBE BASED RAMAN NANOPROBES BY RAMANHYPERSPECTRAL IMAGING: MULTIPLEXING AND BIODETECTION

The potential ofPhoton etc. Raman Imaging Platform, RIMA?, was demonstrated byPr. R Martel’s group at Université de Montréal in a recent publication in Nature Photonics on the developmentof Raman nanoprobes [1].

These new kind of nanoprobes are based on single-wall carbon nanotubesand J-aggregated dyes, such as α?sexithiophene (6T), β-carotene (βcar) andphenazine (Ph). Compared to fluorescent probes, Raman probes have theadvantages of being more stable over long periods of times (weeks and years)and they produce a unique signature with narrow peaks that allows easymultiplexing of 3 probes or more using the same excitation laser energy. Thisnanomaterial shows a very high Raman scattering cross-section, without anyphotobleaching or fluorescence background, even at high laser intensities.

In this work RIMA? enabled the imaging and multiplexing ofthree different probes with sensitivity down to the single object as seen inFigure 1.  The different probes were deposited on a SiOx/Si surface andcharacterized by taking a single hyperspectral image. We were able todetermine, without a doubt, the position of each isolated probe (diameters: 1.3± 0.2 nm), and even identify the co-localized probes (Fig 1b, Ph and βcar). Thesensitivity, efficiency and hyperspectral properties of RIMA? were essential tothe development of these probes.

The carbon nanotube, which serves as a capsule for the probe, can becovalently functionalized to selectively target biomolecules, such asstreptavidin. We demonstrated RIMA?’s potential in the detection of probesin a biological context by imaging the βcar probe functionalized withPEG-biotin groups that targeted streptavidin.

A pattern of 10 μm spots of streptavidin was created by microcontactprinting and then incubated with the probes. The pattern was maintained hydratedunder a cover slip during imaging and the probes were detected wherestreptavidin was located. Figure 2 shows Raman hyperspectral images at 38000px-1 of two printed surfaces, where streptavidin was deposited eitherinside (main figure) or around the dots (inset). With a single acquisition, asample area of 133 x 133 μm2 was studied using RIMA? with laser excitationat 532 nm. Damages to the samples were also limited due to a uniformillumination over the portion of the sample in the field of view. In terms ofspectral resolution and large surface area imaged, RIMA? providedhyperspectral images in a much shorter time then conventional point-by-pointmapping Raman imagers.

Raman hyperspectral imaging is a powerful technique to study a widerange of materials, from nanopatterned surfaces to biological systems. Becauseof its high throughput, RIMA? allows the acquisition of spectrallyresolved maps of large area samples, without damaging the surface.

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