10-901-00-18,CYTOOCHIPSSTARTERNEUROAX18

PROTOCOLS

PRO-Ext-07_7 CytooStarters Quick User Guide.pdf

PUBLICATIONS

Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis

Hurtado L / Caballero C / Gavilan MP / Cardenas J / Bornens M / Rios RM

keywords:

pericentrosomal golgi ribbon organization, akap450 n-terminal fragments, microtubule nucleation, centrosomal activity, golgi apparatus positioning, directional cell migration, ciliogenesis, y-shaped micropatterned coverslips, cell movement

J. Cell Biol. 2011;193(5):917–933.

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Spatial organization of the extracellular matrix regulates cell-cell junction positioning

Tseng Q / Duchemin-Pelletier E / Deshiere A

keywords:

intercellular junctions, cell line, extracellular matrix

Proc. Natl. Acad. Sci. U.S.A. 2012;109(5):1506–1511.

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A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells

Burnette DT / Shao L / Ott C / Pasapera AM / Fischer RS / Baird MA / Der Loughian C / Delanoe-Ayari H / Paszek MJ / Davidson MW / Betzig E / Lippincott-Schwartz J

keywords:

live-cell microscopy, contractility, 3d structures

J Cell Biol. 2014 Apr 14;205(1):83-96. doi: 10.1083/jcb.201311104. Epub 2014 Apr 7.

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A Mechanical Checkpoint Controls Multicellular Growth through YAP/TAZ Regulation by Actin-Processing Factors

Aragona M / Panciera T / Manfrin A

keywords:

yap/taz, oncology, cell shape, cell proliferation

Cell. 2013; Aug 15 [epub ahead of print].

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A method to recapitulate early embryonic spatial patterning in human embryonic stem cells.

Warmflash A / Sorre B / Etoc F / Siggia ED / Brivanlou AH.

keywords:

hesc, assay, stem cells, developmental biology, maturation, differentiation, gastrulation, spacial patterning

Nat Methods. 2014 Jun 29. doi: 10.1038/nmeth.3016

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A Novel Organelle Map Framework for High-Content Cell Morphology Analysis in High Throughput

Schauer K / Grossier J-P / Duong T

keywords:

organelle map, high content screening, high throughput screening

J Biomol Screen. 2013; Aug 16 [epub ahead of print].

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A review of microfabrication and hydrogel engineering for micro-organs on chips

Verhulsel M / Vignes M / Descroix S / Malaquin L / Vignjevic DM / Viovy J-L

keywords:

tissue engineering, 3d cell culture

Biomaterials. 2013; Dec 04 [epub ahead of print].

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Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity

Théry M / Racine V / Piel M

keywords:

cell polarity, extracellular matrix, morphogenesis, cell normalization, organelle positioning

Proc. Natl. Acad. Sci. U.S.A. 2006;103(52):19771–19776.

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Arl8 and SKIP act together to link lysosomes to kinesin-1

Rosa-Ferreira C / Munro S

keywords:

lysosomes, kinesin

Dev. Cell. 2011;21(6):1171–1178.

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Assessing Kinetics from Fixed Cells Reveals Activation of the Mitotic Entry Network at the S/G2 Transition

Karen Akopyan / Helena Silva Cascales / Elvira Hukasova / Adrian T. Saurin / Erik Müllers / Himjyot Jaiswal / Danielle A.A. Hollman / Geert J.P.L. Kops / René H. Medema / Arne Lindqvist

keywords:

cell cycle, cell division, mitosis

Molecular Cell - 27 February 2014

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Carminomycin I is an apoptosis inducer that targets the Golgi complex in clear cell renal carcinoma cells

Woldemichael GM / Turbyville TJ / Linehan WM / McMahon JB

keywords:

high-throughput screening assays, clear cell renal cell carcinoma (ccrcc), von hippel-lindau (vhl) tumor suppressor gene, golgi sequestration, p-gp-mediated localization of anticancer compounds, disk and l shaped fibronectin micropattern

Cancer Res. 2011;71(1):134–142.

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CD81 regulates cell migration through its association with Rac GTPase

Tejera E / Rocha-Perugini V / López-Martín S

keywords:

cell migration, tetraspanin, rho gtpases; focal adhesions

Mol. Biol. Cell. 2012; Dec 05 [epub ahead of print].

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Cell confinement controls centrosome positioning and lumen initiation during epithelial morphogenesis

Rodrigues-Fraticelli AE / Auzan M / Alonso MA / Bornens M / Martin-Belmonte F

keywords:

morphogenesis, cell normalization

J Cell Biol. 2012;198(6):1011-1023.

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Cell distribution of stress fibres in response to the geometry of the adhesive environment

Théry M / Pépin A / Dressaire E / Chen Y / Bornens M

keywords:

pattern geometry, apices of cell shape, stress fibres, focal adhesions, tractional forces, cell shape, cell surface, cell adhesion, cell line

Cell Motil. Cytoskeleton. 2006;63(6):341–355

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Cell shape and contractility regulate ciliogenesis in cell cycle-arrested cells

Pitaval A / Tseng Q / Bornens M / Théry M

keywords:

primary cilium, cilia, cell shape, cell polarity

J. Cell Biol. 2010;191(2):303–312.

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Cells in tight spaces: the role of cell shape in cell function

Shah JV

keywords:

cell shape, cell function, cell physiological phenomena, ciliogenesis

J. Cell Biol. 2010;191(2):233–236.

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Chapter 7 - Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D Systems

Rodríguez-Fraticelli AE / Martin-Belmonte F

keywords:

3d cell culture, morphogenesis

Methods in Cell Biology.Vol Volume 118. Methods for Analysis of Golgi Complex Function. Academic Press; 2013:105–123.

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Chapter 7 - Studying Mitochondria and Microtubule Localization and Dynamics in Standardized Cell Shapes

Pelikan A / Sillibourne J / Miserey-Lenkei S / Carlier-Grynkorn F / Goud B / Tran PT

keywords:

mitochondria, microtubules, cell shape

Methods in Cell Biology. Vol 115. Academic Press; 2013:97–108.

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Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation

Kiyomitsu T / Cheeseman IM

keywords:

spindle orientation on l-patterned fibronectin-coated micropatterns, rnai experiments on patterned coverslips, dynein-dynactin localization, polo-like kinase 1, numa, lgn, rangtp

Nat. Cell Biol. 2012;14(3):311–317.

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Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in Anaphase

Kiyomitsu T / Cheeseman IM

keywords:

cell division, dynein, spindle orientation

Cell. 2013;154(2):391–402.

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Cortical dynein is critical for proper spindle positioning in human cells

Kotak S / Busso C / G?nczy P

keywords:

golgi, spindle positioning, dynein

J Cell Biol. 2012;199(1):97.

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Directional Persistence of Migrating Cells Requires Kif1C-Mediated Stabilization of Trailing Adhesions

Theisen U / Straube E / Straube A

keywords:

cell migration, kif1c, transport kinesin, directional migration

Dev Cell. 2012;23(6):1153–1166.

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Dynamics of Cell Shape and Forces on Micropatterned Substrates Predicted by a Cellular Potts Model

Philipp J. Albert and Ulrich S. Schwarz

keywords:

forces, dynamics, biophysics, modelling, cell behavior, spreading, prediction

Biophys J. 2014 Jun 3;106(11):2340-52. doi: 10.1016/j.bpj.2014.04.036.

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Experimental and theoretical study of mitotic spindle orientation

Théry M / Jiménez-Dalmaroni A / Racine V / Bornens M / Jülicher F

keywords:

mitotic spindle orientation, cell microenvironment geometry, cortical cues, spindle pole, symmetric/ asymmetric orientation

Nature. 2007;447(7143):493–496.

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FAK transduces extracellular forces that orient the mitotic spindle and control tissue morphogenesis

Nicoletta I. Petridou& Paris A. Skourides

keywords:

mitotic spindle, morphogenesis, fak, development, embryo, mitosis

Nature Communications 5, Article number: 5240 doi:10.1038/ncomms6240

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Filamin A-Hinge region 1-EGFP : a novel tool for tracking the cellular function of filaminA in real-time

Planagumà J / Minsaas L / Pons M / Myhren L / Garrido G / Aragay AM

keywords:

cellular function, filamin a, cell normalization

Plos One. 2012;7(8):e40864.

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Filamin A-hinge region 1-EGFP: a novel tool for tracking the cellular functions of Filamin A in real-time

Planagumà J / Minsaas L / Pons M / Myhren L / Garrido G / Aragay AM

keywords:

filamin a, cell surface, cell physiological phenomena

PLoS ONE. 2012;7(8):e40864.

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Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure

Diogo Mosqueira / Stefania Pagliari / Koichiro Uto / Mitsuhiro Ebara / Sara Romanazzo / Carmen Escobedo-Lucea / Jun Nakanishi / Akiyoshi Taniguchi / Ornella Franzese / Paolo Di Nardo / Marie José Goumans / Enrico Traversa / Perpetua Pinto-do-ó / Takao Aoyagi / and Giancarlo Forte

keywords:

adult cardiac progenitor cell, substrate nanotopography, mechano-transduction; cardiac differentiation; yap/taz

ACS Nano, 2014, 8 (3), pp 2033–2047 Publication Date (Web): February 2, 2014

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Human Bone Marrow Mesenchymal Stem Cells Regulate Biased DNA Segregation in Response to Cell Adhesion Asymmetry

Freida D / Lecourt S / Cras A

keywords:

stems cells, cell division, cell adhesion

Cell Rep. 2013;Oct 17 [epub ahead of print].

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Improved visualization and quantitative analysis of drug effects using micropatterned cells

Degot S / Auzan M / Chapuis V

keywords:

reference cell, drug profiling, quantitative cell analysis, cell adhesion, cytoskeleton, drug evaluation

J Vis Exp. 2010;(46):pii: 2514.

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Intercellular and extracellular mechanotransduction in cardiac myocytes

Kresh JY / Chopra A

keywords:

adult cardiomyocytes, extracellular matrix, mechanical determinants, cytoskeletal organization, shape remodelling, sarcomere/myofibrillar architectural topography, cell shape-dependent functions, myopathies, myocardial infarctus, heart failure, reverse remodeling, disk-, h-, y-, crossbow-shaped micropatterns, sarcomeric alpha-actinin.

Pflugers Arch. 2011;462(1):75–87.

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Intracellular Electric Field and pH Optimize Protein Localization and Movement

Cunningham J / Estrella V / Lloyd M / Gillies R / Frieden BR / Gatenby R

keywords:

egfr/ mapk pathway, intracellular electric field

PLoS ONE. 2012;7(5):e36894.

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Lack of a Functional VHL Gene Product Sensitizes Renal Cell Carcinoma Cells to the Apoptotic Effects of the Protein Synthesis Inhibitor Verrucarin A

Woldemichael GM / Turbyville TJ / Vasselli JR / Linehan WM / McMahon JB

keywords:

cell proliferation, apoptosis, cytotoxic stress

Neoplasia. 2012;14(8):771–777.

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LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets α-parvin to control cell adhesion and migration

Devallière J / Chatelais M / Fitau J

keywords:

cell migration, cell adhesion, focal adhesions

FASEB J. 2012;26(6):2592–2606.

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Long-Lasting Effect of Perinatal Exposure to L-tryptophan on Circadian Clock of Primary Cell Lines Established from Male Offspring Born from Mothers Fed on Dietary Protein Restriction

Nascimento E / Guzman-Quevedo O / Delacourt N

keywords:

circadian clock, undernutrition, primary cell line, l-tryptophan

PLoS ONE. 2013;8(2):e56231.

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MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis

Samora CP / Mogessie B / Conway L / Ross JL / Straube A / McAinsh AD

keywords:

mitotic spindle orientation, h pattern, cell division, cell line, mitosis, mitotic spindle apparatus, microtubules

Nat. Cell Biol. 2011;13(9):1040–1050.

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Mechanical control of epithelial lumen formation

Rodríguez-Fraticelli AE / Martin-Belmonte F

keywords:

epithelial cells, lumen, ciliogenesis, centrosome, morphogenesis

Small GTPases. 2013;4(2).

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Mechanism of the Ca2+-Dependent Interaction between S100A4 and Tail Fragments of Nonmuscle Myosin Heavy Chain IIA.

Badyal SK / Basran J / Bhanji N

keywords:

epidermoid carcinoma a431/sip1 cells constitutively expressing the gfp-tagged heavy chain of non-muscle myosin iia, medium y-shaped fibronectin micropattern

J Mol Biol. 2011;405(4-3):1004–1026.

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Mechanisms of spindle positioning: cortical force generators in the limelight

Kotak S / G?nczy P

keywords:

cell division, spindle, microtubules

Curr Opin Cell Biol. 2013;Aug 16 [epub ahead of print].

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Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes

Kwon M / Godinho SA / Chandhok NS

keywords:

tumor cells, micropatterns, h shape, y shape, cell division, neoplasms, cell adhesion, cell line, cell shape, cell polarity

Genes Dev. 2008;22(16):2189–2203.

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Microfluidic cell trap array for controlled positioning of single cells on adhesive micropatterns

Lin L / Chu Y-S / Thiery JP / Lim CT / Rodriguez I

keywords:

cell shape, cell polarity, cell function, cell migration, single cells

Lab Chip. 2013;13(4):714–721.

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Micropatterned substrates coated with neuronal adhesion molecules for high-content study of synapse formation

Cz?nd?r K / Garcia M / Argento A

keywords:

neuroscience, neuron cell, cell adhesion, morphogenesis

Nat Commun. 2013;4(2252).

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Micropatterning as a tool to decipher cell morphogenesis and functions

Théry M.

keywords:

cell microenvironment, cell architecture, engineered micropatterns, cell morphogenetic processes, micropattern shape, actin, microtubule, cell differentiation, cell polarity, cell shape, cell proliferation

J. Cell. Sci. 2010;123(24):4201–4213.

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Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns

Stiles JM / Pham R / Rowntree RK

keywords:

endothelial cells, cell morphology, cytoskeleton, genomics

FEBS J. 2013; June 27 [epub ahead of print].

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Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation

Guenin-Macé L / Veyron-Churlet R / Thoulouze M-I

keywords:

immunology, buruli, mycolactone

J Clin Invest. 2013; Mar 15 [epub ahead of print].

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Myosin-Va and Dynamic Actin Oppose Microtubules to Drive Long-Range Organelle Transport

Richard D. Evans / Christopher Robinson / Deborah A. Briggs / David J. Tooth / Jose S. Ramalho / Marta Cantero / Lluis Montoliu / Shyamal Patel / Elena V. Sviderskaya / and Alistair N. Hume

keywords:

microtubule, actin, myosin, melanosomes, melanocytes, reference cell, organelles, trafficking

Curr Biol. 2014 Aug 4;24(15):1743-50. doi: 10.1016/j.cub.2014.06.019. Epub 2014 Jul 24.

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Patterning Pluripotent Stem Cells at a Single Cell Level

Pryzhkova MV / Harris GM / Ma S / Jabbarzadeh E

keywords:

stem cells, vitronectin, extracellular matrix

J Biomater Tissue Eng. 2013;3(4):461–471

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Phosphatidylinositol 5-phosphate regulates invasion through binding and activation of Tiam1

Julien Viaud / Frederic Lagarrigue / Damien Ramel / Sophie Allart / Gaetan Chicanne / Laurie Ceccato / Delphine Courilleau / Jean-Marie Xuereb / Olivier Pertz / Bernard Payrastre & Frederique Gaits-Iacovoni

keywords:

phosphoinositides, oncology, rpe-1, mef, invasion, bacteriology, lymphoma, fret, endosome

Nat Commun. 2014 Jun 6;5:4080. doi: 10.1038/ncomms5080.

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Practical course on “Imaging infection: from single molecules to animals.”

Guenot M / Racz P

keywords:

fluorescence microscopy, bioluminescent imaging, high throughput microscopy, super-resolution microscopy : tirf, palm/storm, in vivo imaging, petscan, ct-scan, fret, live cell imaging, image analysis, photobleaching, phototoxicity

Microbes Infect. 2012; Nov 02 [epub ahead of print].

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Probabilistic density maps to study global endomembrane organization

Schauer K / Duong T / Bleakley K / Bardin S / Bornens M / Goud B.

keywords:

endomembranes, small g proteins, cell line, intracellular membranes

Nat. Methods. 2010;7(7):560–566.

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Rab GTPase Mediated Procollagen Trafficking in Ascorbic Acid Stimulated Osteoblasts

Nabavi N / Pustylnik S / Harrison RE

keywords:

osteoblasts, protein trafficking

PLoS ONE. 2012;7(9):e46265.

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Reconstituting actomyosin-dependent mechanosensitive protein complexes in vitro

Ciobanasu C / Faivre B / Le Clainche C

keywords:

mechanosensitivity, abp, actomyosin, cytoskeleton, vinculin, talin, tirf

Nat Protoc. 2015 Jan;10(1):75-89. doi: 10.1038/nprot.2014.200. Epub 2014 Dec 11.

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Reconstitution of in vivo macrophage-tumor cell pairing and streaming motility on one-dimensional micro-patterned substrates

Sharma V / Beaty B / Patsialou A

keywords:

cell migration, oncology

IntraVital. 2012;1(1):77-85.

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Reprogramming cell shape with laser nano-patterning.

Vignaud T / Galland R / Tseng Q / Blanchoin L / Colombelli J / Théry M.

keywords:

cell shape, actin network reprogramming, dynamic cellular responses, laser nano-patterning, cell polarity

J. Cell. Sci. 2012;125(9):2134–2140.

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Role of YAP/TAZ in mechanotransduction

Dupont S / Morsut L / Aragona M

keywords:

mechanotransduction, mesenchymal stem cells, cell differentiation, cell line, cell shape, cytoskeleton

Nature. 2011;474(7350):179–183.

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Spatial control of membrane receptor function using ligand nanocalipers

Alan Shaw / Vanessa Lundin / Ekaterina Petrova / Ferenc F?rd?s / Erik Benson / Abdullah Al-Amin / Anna Herland / Andries Blokzijl3 / Bj?rn H?gberg & Ana I Teixeira

keywords:

signaling, receptor, ligand, nanocaliper, breast cancer, membrane, ephrin-a5, invasion, presentation

Nat Methods. 2014 Aug;11(8):841-6. doi: 10.1038/nmeth.3025. Epub 2014 Jul 6.

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Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL

Kitagawa D / Kohlmaier G / Keller D

keywords:

microcephaly, mitotic spindle orientation

J. Cell. Sci. 2011;124(22):3884–3893.

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Standardized mitochondrial analysis gives new insights into mitochondrial dynamics and OPA1 function. Int

Chevrollier A / Cassereau J / Ferré M

keywords:

mitochondrial dynamics, mitochondrial disorder diagnostics, opa1, neurodegenerative diseases, mitochondria

J. Biochem. Cell Biol. 2012;44(6):980–988.

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The Bin/Amphiphysin/Rvs (BAR) Domain Protein Endophilin B2 Interacts with Plectin and Controls Perinuclear Cytoskeletal Architecture

Vannier C / Pesty A / San-Roman MJ / Schmidt AA

keywords:

cytoskeleton, nucleus positioning

J Biol Chem. 2013;288(38):27619–27637.

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The extracellular matrix guides the orientation of the cell division axis.

Théry M / Racine V / Pépin A

keywords:

cell fate, cell division, cell shape, cell membrane, cell polarity, actin dynamics, average distribution of actin-binding proteins

Nat. Cell Biol. 2005;7(10):947–953.

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The interaction between nesprins and sun proteins at the nuclear envelope is critical for force transmission between the nucleus and cytoskeleton.

Lombardi ML / Jaalouk DE / Shanahan CM / Burke B / Roux KJ / Lammerding J.

keywords:

cell line, cytoskeleton, mechanotransduction, nuclear envelope, nucleo-cytoskeletal coupling, force transmission, linc (linker of nucleoskeleton and cytoskeleton) complex, intracellular forces, nuclear positioning, cell polarization, muscular dystrophies, cardiomyopathies, crossbow-shaped micropatterns

J. Biol. Chem. 2011;286(30):26743–26753.

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The role of RPGR in cilia formation and actin stability

Gakovic M / Shu X / Kasioulis I / Carpanini S / Moraga I / Wright AF

keywords:

cilia, ciliogenesis, retinal dystrophy, actins, cytoskeleton, cell cycle, cell division, cell line, cell movement, cell shape, epithelial cells, eye proteins, focal adhesions, organogenesis

Hum. Mol. Genet. 2011;20(24):4840–4850

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β1- and αv-class integrins cooperate to regulate myosin II during rigidity sensing of fibronectin-based microenvironments

Schiller HB / Hermann M-R / Polleux J

keywords:

integrins, cell adhesion, cell contractility, cell motility, traction force

Nat Cell Biol. 2013; May 26 [epub ahead of print] .

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SHIPMENT AND INFORMATION

In order to guarantee a constant quality as well as a long shelf life for our products, CYTOOchips are packaged by 6 in a blister pack sealed in an aluminum bag under inert gas.

The average turnaround time for production is 4-5 weeks.

The CYTOOchips should be stored at 4°C and used before the expiry date indicated on the packaging (6 months from the production date).

Stability is guaranteed for at least 3 months from the date of shipment when stored at 4°C in its original packaging.

10-901-00-18,CYTOOCHIPSST…

CYTOOCHIPS? STARTER NEURO A X18

Product specification

PROTOCOLS

PUBLICATIONS

SHIPMENT AND INFORMATION