Seahorse Bioscience XFp Analyzer
The XFp Analyzer features fast and easy setup using built-in assay templates and a miniplate format, making it ideal for researchers who run just a few samples per day. Key applications include metabolic phenotyping of rare cell types, patient-derived samples, and other precious cells. The XFp Analyzer is our most affordable XF platform and is suitable for performing our gold standard Stress Tests in a pairwise comparison format.
Seahorse Bioscience XFe96 Analyzer
Seahorse Bioscience The XFe96 Analyzer provides the highest capacity for XF assays at the lowest per-sample cost. The XFe96 enables a large number of experimental groups per assay, making it ideal for labs seeking to maximize design flexibility and sample throughput. Recommended for model organisms, screening applications as well as dose-response studies. Can analyze individual spheroids with the XFe96 Spheroid Micoplate. Validated for assays performed in a hypoxia chamber.
Seahorse Bioscience XFe24 Analyzer
The XFe24 is validated by hundreds of peer-reviewed publications.
Selection Guide
| XFp
Analyzer | XFe96
Analyzer | XFe24
Analyzer |
|
| Plate Format | Miniplate | Microplate | Microplate |
| Number of Assay Wells | 6 | 96 | 24 |
| Microchamber volume | 2 ?L | 2 ?L | 7 ?L |
| Assay Working Volume | 150-275 ?L | 150-275 ?L | 500-1000 ?L |
| Recommended Injection Volume | 25 ?L | 25 ?L | 75 ?L |
| Controller | Integrated full-color touch-screen interface to design basic protocols and run imported templates. | Combination computer and touchscreen display with full assay design, control, and analysis capability. | Combination computer and touchscreen display with full assay design, control, and analysis capability. |
| Software | Create and run standard assays on controller OR
design on Wave desktop, transfer to Controller, return to desktop for analysis. | Design and analyze assay templates on Controller or Wave desktop. | Design and analyze assay templates on Controller or Wave desktop. |
| Best For | - Pairwise comparisons
- Precious biomaterial
- Patient-derived samples
| - Testing many conditions at one time
- Dose-response studies
- Spheroids
- Model organisms
| |
| Key Advantages | - Easy to set up and run via streamlined interface
- Standardized protocols for XF Stress Tests
| - Maximum experimental flexibility
- Highest throughput
- Validated for hypoxia
| |
All XF Analyzers Feature:
- Capacity to perform Gold Standard XF Assays including the Cell Mito Stress Test, Glycolysis Stress Test, and Metabolic Switching
- Compatibility with both adherent and suspension cells as well as isolated mitochondria.
- Ability to perform up to 4 independent injections per well with automatic mixing
- Simultaneous measurement of OCR and ECAR in the same well
- Sensitivity for small sample sizes
- Label-free detection in real time
- Automatic calculation of oxygen consumption rate and extracellular acidification rate
- Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data
Cell Lines and Seeding Density
| Cell Line | Seeding Density Range of Cells/Well |
| XFp/XFe96 | XFe24 |
| 3T3-L1 - Preadipocytes | 5-7x103 | 10-40x103 |
| A549 Cells | 4x104 | 2x104 |
| Astrocytes | 5x103 - 20x103 | 50x103 - 100x103 |
| BMDM | 8x104 | 10-50x104 |
| C2C12 | 8x104 | 5x104 |
| Cardiomyocytes (primary) | 3-5x104 | 4x104 - 15x104 |
| Cortical Neurons (primary) | 1-4x104 | 5x104 - 10x104 |
| Dermal Fibroblasts | 2-4x104 | 1-5x104 |
| H9C2 | 1-2x104 | 2-5x104 |
| HCT116 | 1-2x104/ | 3-5x104 |
| HEK 293 | 2-5x104/ | 5x104 |
| HeLa | 1-3x104 | 1-5x104 |
| Hepatocytes (primary) | 1x104 | 1-4x104 |
| HepG2 | 1-3x104 | 4-5x104 |
| HUVEC | 1x104/ | 2-3x104 |
| iPSC | 5x103 | 50-70x103 |
| Isolated mitochondria | 1-5 ?g/well | 2-10 ?g/well |
| Jurkat | 1x105 | 1-2x105 |
| MCF10A | 1-3x104/ | 2-4x104 |
| MCF7 | 2x104 | 2-4x104 |
| MDA-MB-231 | 1-2x104 | 3-5x104 |
| MEF | 1x104/ | 1-4x104 |
| PC12 | 8x104 | 5x104 |
| RAW 264.7 | 8x104 | 6x104 - 20x104 |
| T-cells (primary) |