PRODUCT DESCRIPTION产品描述
E.coli RNase H (rnh) is an endoribonucleasewhich degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestionproduces ribonucleotide molecules with 5-phosphate and 3-hydroxyl termini.RNAse H is nearly inactive against single or double-stranded RNA molecules.
Source of Protein蛋白来源
A recombinant E. coli strain carrying theRNAse H (rnh) gene from E.coli.
Supplied in
20 mM Tris-HCl
100 mM KCl
10 mM MgCl2
0.1 mM EDTA
0.1 mM DTT
50% glycerol
pH 7.9 @ 25°C
Supplied With
B9220 (10X RNAse H Buffer)
10X RNAse H Buffer (B9220):
500 mM Tris-HCl
750 mM KCl
30 mM MgCl2
100 mM DTT
pH 8.3 @ 25°C
Unit Definition单位定义
1 unit is defined as the amount of enzymethat will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid moleculeinto acid-soluble material in 20 minutes at 37°C.
PRODUCT INFORMATION
RNAse H
Part Number Y9220L, Y9220F
Concentration 5,000 U/ml
Unit Size 5,000 U, 770U
SDS Availableon request
PRODUCT SPECIFICATION*产品特性
Storage Temperature -25 to -15°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 625,000 U/mg
SS Exonuclease 500 U <5.0%released
DS Exonuclease 500 U <1.0%released
DS Endonuclease 500 U = Noconversion
E.coli DNA Contamination 500 U <10 copies
RNAse Contamination 500 = No Detectable non-specific RNase
* For a detailed summary of assayconditions and data, refer to the Quality Controls Analysis section.
Limitations of Use适用范围
This product was developed, manufactured,and sold for in vitro use only. The product is not suitable for administrationto humans or animals.
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