PRODUCT DESCRIPTION产品描述
Taq DNA Ligase catalyzes the formation of aphosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+as a cofactor.
Source of Protein蛋白来源
A recombinant E. coli strain carrying thecloned Taq DNA Ligase gene.
Supplied in
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
0.1% Tween-20
50% glycerol
pH 7.5 @ 25°C
Supplied With
B6060 (10X Taq DNA Ligase Buffer)
10X Taq DNA Ligase Buffer (B6060)
200 mM Tris-HCI
250 mM KCl
100 mM MgCl2
5 mM NAD
0.1% Triton X-100
pH 7.6 @ 25°C
Unit Definition单位定义
1 unit is defined as the amount of Taq DNALigase required to join 50% of 1 μg of the 12-base cohesive ends of Lambda DNAcut with Sma I and Sal I in 50 μl 1X Taq DNA Ligase Buffer following a 10minute incubation at 45°C.
PRODUCT INFORMATION产品信息
Taq DNA Ligase
Part Number L6060L L6060F
Concentration 40,000 U/ml
Unit Size 20,000 U 3,100 U
SDS Available on request
PRODUCT SPECIFICATION*产品特性
Storage Temperature -25 to -15°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 400,000 U/mg
SS Exonuclease 400 U <5.0%released
DS Exonuclease 400 U <1.0%released
DS Endonuclease 400 U = Noconversion
E.coli DNA Contamination 400U <10 copies
* For a detailed summary of assayconditions and data, refer to the Quality Controls Analysis section.
Limitations of Use适用范围
This product was developed, manufactured,and sold for in vitro use only. The product is not suitable for administrationto humans or animals.
www.hyqbio.com.cn