PRODUCT DESCRIPTION产品描述
T7 DNA Ligase catalyzes the formation of aphosphodiester bond between a 5′phosphate and a 3′hydroxyl termini in duplex DNA. The enzyme will join blunt end andcohesive end termini as well as repair single stranded nicks in duplex DNA.
Source of Protein蛋白来源
A recombinant E. coli strain carrying theT7 DNA Ligase gene.
Supplied in
20 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
50% glycerol
pH 7.5 @ 25°C
Supplied With:
B1010 (2X Rapid Ligation Buffer)
2X Rapid Ligation Buffer (B1010):
132mM Tris-HCI
20 mM MgCl2
2 mM DTT
2 mM ATP
15% PEG 6000
pH 7.6 @ 25°C
Unit Definition 单位定义
1 unit is defined as the amount of T7 DNALigase required to ligate 50% of 100 ng DNA fragments with cohesive termini in30 minutes at 23°C.
PRODUCT INFORMATION产品信息
T7 DNA Ligase
Part Number L6020L, L6020F
Concentration 3,000,000 U/ml
Unit Size 900,000 U, 138,000
SDS Available on request
PRODUCT SPECIFICATION*产品特性
Storage Temperature -25 to -15°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 3,000,000 U/mg
SS Exonuclease 30,000 U <1.0%released
DS Exonuclease 30,000 U <1.0%released
DS Endonuclease 30,000 U = Noconversion
E.coli DNA Contamination 30,000 U <10 copies
* For a detailed summary of assayconditions and data, refer to the Quality Controls Analysis section.
NOTES
In the absence of high concentrationcrowding agents (eg 20-30% PEG 6-8000), the specific activity of T7 DNA Ligaseis reduced by 1000-fold on blunt-ended fragments (1, 2).
T7 DNA Ligase is incapable of ligatingsingle-stranded DNA fragments (1).
REFERENCES参考文献
Doherty, A. et al. J.Biol. Chem (1996)V.271, No.19, 11083-11089.
Dayal, T. Unpublished observations.
Limitations of Use适用范围
This product was developed, manufactured,and sold for in vitro use only. The product is not suitable for administrationto humans or animals.