PRODUCT DESCRIPTION产品描述
T4 RNA Ligase 2 truncated catalyzesphosphodiester bond formation between a pre-adenylated 5′phosphate (DNA or RNA) and the 3′hydroxyl of RNA. The truncatedenzyme contains the first 249 amino acids which makes the enzyme require apre-adenylated 5′terminaldonor and eliminates the need for ATP. Because T4 RNA ligase 2 truncated cannotuse the 5′phosphate ofRNA or DNA as a donor in the ligation reaction, it is useful for certainapplications such as linker ligations with pre-adenylated 5′DNA to 3′hydroxyl RNA. The desired specificligation products are enhanced dramatically over unwanted background ligationproducts, making the truncated enzyme superior to the full-length enzyme forthis use.
Source of Protein蛋白来源
Purified from a strain of E. coli thatexpresses the recombinant truncated T4 RNA Ligase 2 gene.
Supplied in
10 mM Tris-HCl
100 mM NaCl
0.1 mM DTT
0.1 mM EDTA
50% glycerol
pH 7.5 @ 25°C
Supplied With:
B6070 10X T4 RNA Ligase 2, Truncated Buffer
10X T4 RNA Ligase 2, Truncated Buffer(B6070)
500 mM Tris-HCl
100 mM MgCl2
50 mM DTT
pH 7.6 @ 25°C
Unit Definition单位定义
One unit is defined as the amount of enzymerequired to ligate 50% of 0.4 μg of an equimolar mix of a single-stranded 5′ FAM-labeled 17-mer RNA to the 5′ pre-adenylated end of a 18-mer DNAwhen both 17-mers are annealed to a complementary 35-mer DNA strand in 20 μL 1Xreaction buffer following a 30 minute incubation at 37°C.
PRODUCT INFORMATION产品信息
T4 RNA Ligase 2 Truncated
Part Number L6070L
Concentration 5,000 U/ml
Unit Size 500 U
SDS Available on request
PRODUCT SPECIFICATION*产品特性
Storage Temperature -25 to -15°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 30,000 U/mg
SS Exonuclease 50 U <5%released
DS Exonuclease 50 U <1%released
DS Endonuclease 50 U = No conversion
E.coli DNA Contamination 50 U< 10 copies
RNAse Contamination 50 U = No detectable non-specific RNAse
* For a detailed summary of assayconditions and data, refer to the Quality Controls Analysis section.
NOTES
Enzymatics’T4 RNA Ligase 2 Truncated demonstrates equivalent or superiorvolume/volume performance when compared to analogous T4 RNA Ligase 2 Truncatedproducts on the market.
REFERENCES参考文献
Ho, C.K. et al. (2004) Structure, 12,327-339.
Ho, C.K. and Shuman, S. (2002) Proc.Natl.Acad.Sci. USA, 99, 12709-12714.
Nandakumar, J. et al. (2004) J. Biol. Chem,279, 31337-31347.
Aravin, A. and Tusch, T. (2005) FEBSLetters, 579, 5830-5840.
Pfeffer, S. et al. (2005) Nat. Meth, 2,269-276.
Limitations of Use适用范围
This product was developed, manufactured,and sold for in vitro use only. The product is not suitable for administrationto humans or animals.
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