PRODUCT DESCRIPTION产品描述
T4 RNA Ligase catalyzes the ATP-dependentligation of single-stranded nucleic acids (RNA or DNA).
Source of Protein蛋白来源
A recombinant E. coli strain carrying theT4 RNA Ligase gene from bacteriophage T4.
Supplied in
10 mM Tris-HCl
50 mM KCl
0.1 mM EDTA
1.0 mM DTT
50% glycerol
pH 7.5 @ 25°C
Supplied With
B6050 (10X T4 RNA Ligase Buffer)
10X T4 RNA Ligase Buffer (B6050)
500 mM Tris-HCl
100 mM MgCl2
10 mM ATP
100 mM DTT
pH 7.8 @ 25°C
Unit Definition单位定义
One unit is defined as the amount of enzymerequired to ligate 50% of 0.4 μg of an equimolar mix of two single stranded 23base RNA oligonucleotides (one 5′-phosphorylated) in 20 μL 1X T4 RNA Ligase Buffer following a 30minute incubation at 37°C.
PRODUCT INFORMATION产品信息
T4 RNA Ligase 1
Part Number L6050L, L6050F
Concentration 20,000 U/ml
Unit Size 10,000 U,1,540 U
SDS Available on request
PRODUCT SPECIFICATION*产品特性
Storage Temperature -25 to -15°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 16,800 U/mg
SS Exonuclease 200 U <5.0%released
DS Exonuclease 200 U <1.0%released
DS Endonuclease 200 U = No conversion
E.coli DNA Contamination 200 U <10 copies
Non-specific RNAse 200 U none detected
* For a detailed summary of assay conditionsand data, refer to the Quality Controls Analysis section.
Limitations of Use适用范围
This product was developed, manufactured,and sold for in vitro use only. The product is not suitable for administrationto humans or animals.
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