PRODUCT DESCRIPTION产品描述
T7 RNA Polymerase is a DNA-dependent RNApolymerase having high specificity for the T7 promoter. After promoterinitiation, it catalyzes the Mg2+ dependent synthesis of RNA fromrNTPs.
Source of Protein蛋白来源
Purified from a strain of E. coli thatexpresses the recombinant T7 RNA Polymerase gene.
Supplied in
50 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
50% glycerol
0.1% Triton X-100
pH 7.9 @ 25°C
Supplied With
B7180 (10X T7 RNA Polymerase Buffer)
10X T7 RNA Polymerase Buffer (B7180)
400 mM Tris-HCl
60 mM MgCl2
100 mM DTT
20 mM Spermidine
pH 7.9 @ 25°C
Unit Definition单位定义
One unit is defined as the amount of enzymethat will incorporate 1 nmol of ATP into acid-precipitable material in 1 hourat 37°C.
PRODUCT INFORMATION产品信息
T7 RNA Polymerase
Part Number P7180L,P7180F
Concentration 50,000 U/ml
Unit Size 50,000 U, 1,700U
SDS Available on request
PRODUCT SPECIFICATION*产品特性
Storage Temperature -25 to -15°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 312,500 U/mg
SS Exonuclease 500 U <1.0% released
DS Exonuclease 500 U <1.0%released
DS Endonuclease 500 U = No conversion
E.coli DNA Contamination 500 U <10 copies
RNAse Contamination 500 U = No Detectable non-specific RNAse
* For a detailed summary of assayconditions and data, refer to the Quality Controls Analysis section.
REFERENCES参考文献
Chamberlin, M, et al. (1973) J. Biol. Chem.248, 2235-2244, 2245-2250.
Chamberlin, M. et al. (1982) in TheEnzymes, 3rd edition, ed. P. D. Boyer (Academic Press, New York.) 15, 87-108.
Limitations of Use 适用范围
This product was developed, manufactured,and sold for in vitro use only. The product is not suitable for administrationto humans or animals.
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