PRODUCT DESCRIPTION产品描述
T4 DNA Polymerase catalyzes the extensionof a primed DNA template in the 5′→ 3′ direction.This enzyme exhibits a powerful 3′→ 5′ exonucleaseactivity, while lacking any inherent 5′→ 3′ exonucleaseor strand displacement functions.
Source of Protein蛋白来源
Purified from a strain of E. coli thatexpresses the recombinant T4 DNA Polymerase gene.
Supplied in
100 mM KPO4
1.0 mM DTT
0.1 mM EDTA
50% glycerol
pH 6.5 @ 25°C
Supplied With
B0110 (10X Blue Buffer)
10X Blue Buffer (B0110)
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
10 mM DTT
pH 7.9 @ 25°C
Unit Definition单位定义
One unit is defined as the amount of enzymethat will incorporate 10 nmol of dNTP into acid-precipitable material in 30minutes at 37°C.
PRODUCT INFORMATION产品信息
T4 DNA Polymerase
Part Number P7080L
Concentration 3,000 U/ml
Unit Size 2,000U
SDS Available on request
PRODUCT SPECIFICATION*产品特性
Storage Temperature -15 to -25°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 5,555 U/mg
SS Exonuclease Functional
DS Exonuclease Functional
DS Endonuclease 30 U = no conversion
E.coli DNA Contamination 30 U < 10 copies
* For a detailed summary of assayconditions and data, refer to the Quality Controls Analysis section.
REFERENCES参考文献
Tabor, S. and Struhl, K. (1989) InDNA-Dependent DNA Polymerases. F. M. Ausebel, R. Brent, R. E. Kingston, D. D.Moore, J. G. Seidman, J. A. Smith and K. Struhl (Eds.), Current Protocols inMolecular Biology, pp. 3.5.10-3.5.12. 2. Sambrook, J., Fritsch, E.F. andManiatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd Ed.),5.44-5.47.
Dale, R., McClure, B. and Houchins, J.(1985) Plasmid, 13, 31-40.
Kunkel, T.A., Roberts, J.D. and Zakour,R.A. (1987) R. Wu and L. Grossman (Eds.), Methods Enzymol., 154, pp. 367-382.San Diego: Academic Press.
Panet, A., van de Sande, J.H., Loewen, P.C.and Khorana, H.G. (1973) Biochemistry, 12, 5045-5050.
Limitations of Use适用范围
This product was developed, manufactured,and sold for in vitro use only. The product is not suitable for administrationto humans or animals.