PRODUCT DESCRIPTION产品描述
Manta 1.0 DNA Polymerase (exo-) is athermophilic DNA polymerase deficient in both proofreading (3′→ 5′) and nick-translation (5′→ 3′) nuclease activities.The protein was originally characterized and its crystal structure solved byLorena Beese (1).
Source of Protein蛋白来源
A recombinant E. coli strain carrying theManta 1.0 DNA Polymerase (exo-) polymerase gene.
Supplied in
10 mM Tris-HCl
50 mM KCl
1.0 mM DTT
0.1 mM EDTA
0.1% Triton X-100
50% glycerol
pH 7.5 @ 25°C
Supplied With
B7140 (10X PCR Buffer II)
10X PCR Buffer ll (B7140):
200 mM Tris-HCl
100 mM (NH4)2SO4
100 mM KCl
20 mM MgSO4
1.0% Triton X-100
pH 8.8 @ 25°C
Unit Definition单位定义
1 unit is defined as the amount ofpolymerase required to convert 10 nmol of dNTPs into acid insoluble material in30 minutes at 65°C.
PRODUCT INFORMATION产品信息
Manta 1.0 DNA Polymerase (HighConcentration)
Part Number P7140-HC-F, P7140-HC-L
Concentration 400,000 U/ml
Unit Size 15,400 U, 100,000U
SDS Available on request
PRODUCT SPECIFICATION*产品特性
Storage Temperature -25 to -15°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 400,000 U/mg
SS Exonuclease 4000 U <5.0%released
DS Exonuclease 4000 U <1.0%released
DS Endonuclease 4000 U = no conversion
E.coli DNA Contamination 4000 U <10 copies
* For a detailed summary of assayconditions and data, refer to the Quality Controls Analysis section.
NOTES
Specific activity measured under aboveconditions is approximately 3X higher than cited by Beese et al. when usingactivated calf thymus DNA as a substrate.
REFERENCES参考文献
Kiefer, et al. Structure 15 January 1997. 5,95-108.
Limitations of Use适用范围
This product was developed, manufactured,and sold for in vitro use only. The product is not suitable for administrationto humans or animals.