| Designation: | MG-63 | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 14 years of age | | Gender: | Male | | Tissue: | Bone | | Morphology: | Fibroblast-like | | Celltype: | Osteosarcoma | | Growth Properties: | Monolayer | | Description: | High levels of interferon production can be induced using polyinosinic - polycytidylic acid, cycloheximide and actinomycin D. | | Culture Medium: | DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:4 to 1:8 is recommended | | Fluid Renewal: | 2 to 3 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | DNA Profile (STR): | Amelogenin: X / Y
CSF1PO: 10/12
D13S317: 11/11
D16S539: 11/11
D5S818: 11/12
D7S820: 10/10
THO1: 9.3/9.3
TPOX: 8/11
vWA: 16/19
D3S1358: 15/17
D21S11: 28/29
D18S51: 13/13
Penta E: 12/12
Penta D: 11/13
D8S1179: 11/15
FGA: 20/21
CLS ? Cell Lines Service, 2009.
| | Receptors Expressed: | transforming growth factor beta (TGF beta, type I and type II) | | Products: | interferon |
References:
Billiau A et al. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob Agents Chemother 12: 11-5, 1977.
Heremans H et al. In vitro cultivation of human tumor tissues. II. Morphological and virological characterization of three cell lines. Oncology 35: 246-52, 1978.
Clover J et al. Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? Bone 15: 585-591, 1994.
Takeuchi Y et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J Cell Physiol 162: 315-21, 1995.
Yee A et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J Biol Chem 271: 471-477, 1996.
Vial D et al. Coordinate regulation of fibronectin matrix assembly by the plasminogen activator system and vitronectin in human osteosarcoma cells. Cancer Cell Int 6: 8, 2006.
Wiontzek M et al. Effects of dexamethasone and celecoxib on calcium homeostasis and expression of cyclooxygenase-2 mRNA in MG-63 human osteosarcoma cells. Clin Exp Rheumatol 24: 366-72, 2006. |
| HRT-18 (HCT-8) |
| Designation: | KHOS-NP | | Depositor: | Rhim | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 13 years old | | Gender: | female | | Tissue: | Bone | | Celltype: | osteosarcoma | | Growth Properties: | monolayer | | Description: | This cell line was derived from the HOS cell line (TE-85) by transformation using Kirsten murine sarcoma virus (Ki-MSV). The cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice. The cells are useful producing MSV pseudotypes with various ecotropic and xenotropic murine leukemia viruses. Cells carry the Ki-MSV genome but do not produce infectious virus particles or viral antigens. | | Culture Medium: | Minimum essential medium Eagle (Earle's BSS) supplemented with 2 mM L-glutamine, 0.1 mM non-essential amino acids (NEAA), 1.0 mM sodium pyruvate, and 10% fetal bovine serum (MG-10, CLS order number 820100). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:2 to 1:4 is recommended | | Fluid Renewal: | 2 to 3 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | Tumorigenic: | yes, in nude mice | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
D3S1358: 15
D21S11: 31.2,32.2
D18S51: 17
Penta E: 7,12
Penta D: 9,10
D8S1179: 11,14
FGA: 24
CLS ? Cell Lines Service, 2011. | | Viruses: | SMRV negative, as confirmed by Real-Time PCR
|
References:
Rhim JS et al. Non-producer human cells induced by murine sarcoma virus. Int J Cancer 15: 23-9, 1975.
Rhim JS et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int J Cancer 16: 840-9, 1975.
Ganjavi H et al. Adenovirus-mediated p53 gene therapy in osteosarcoma cell lines: sensitization to cisplatin and doxorubicin. Cancer Gene Ther 13: 415-9, 2006.
Izbicka E et al. Molecular determinants of differential sensitivity to docetaxel and paclitaxel in human pediatric cancer models. Anticancer Res 26: 1983-8, 2006. |
| Sobrero |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 67 years; |
| Gender: | male |
| Tissue: | colon |
| Morphology: | epithelial |
| Celltype: | colorectal adenocarcinoma, ileocecal |
| Growth Properties: | monolayer |
| Description: | The HCT-8 line is identical to the HRT-18 cell line. The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
D3S1358: 17
D21S11: 29,32.2
D18S51: 11,17
Penta E: 7,14
Penta D: 9,14
D8S1179: 15
FGA: 22
CLS ? Cell Lines Service, 2011. |
| Isoenzymes: | AK-1, 1; ES-D, 1-2; GLO-1, 2; G6PD, B; PGM1, 1; PGM3, 1; Me-2, 1 |
| Reverse Transcriptase: | negative |
| Products: | carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin |
References:
Tompkins WA et al. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. Cancer Inst. 52: 1101-1110, 1974.
Nelson-Rees WA et al. Distinctive banded marker chromosomes of human tumor cell lines. Int. J. Cancer 16: 74-82, 1975.
White LJ et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996.
Lee JY, Hwang WI, Lim ST. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J Ethnopharmacol 93(2-3):409-15, 2004.
Kobel M, Budianto D, Schmitt WD, Borsi L, Siri A, Hauptmann S. Influence of various cytokines on adhesion and migration of the colorectal adenocarcinoma cell line HRT-18. Oncology 68(1):33-9, 2005. Epub 2005 Mar 2.
Jansen M, Jansen PL, Otto J, Kirtil T, Neuss S, Treutner KH, Schumpelick V. The inhibition of tumor cell adhesion on human mesothelial cells (HOMC) by phospholipids in vitro. Langenbecks Arch Surg 391(2):96-101, 2006. Epub 2006 Mar 14. |