| Designation: | SaOS-2 | | Depositor: | Trempe | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 11 years old | | Gender: | female | | Tissue: | Bone | | Morphology: | Epithelial-like | | Celltype: | Osteosarcoma | | Growth Properties: | adherent | | Description: | The SaOS-2 cell line was established by J. Fogh in 1973. | | Culture Medium: | DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:2 to 1:4 is recommended | | Fluid Renewal: | 2 to 3 times per week | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | Tumorigenic: | no | | Karyotype: | The stemline chromosome number is hypotriploid with the modal number of 56 chromosomes per cell and the 2S component occurring at 13.2%. Over two-thirds of the chromosome complement consisted of structurally rearranged chromosomes. Most marker chromosomes had complex rearrangements. The origin of the segments composing these markers could not be identified. Of the identifiable markers, 6p+/q+, 7p+, 11p+, and 12p+ occasionally were present at 2 copies per cell. The Y chromosome was not detected in the QM stained preparation. | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 10
D13S317: 12, 13
D16S539: 12, 13
D5S818: 12
D7S820: 8, 10
THO1: 6, 9
TPOX: 8
vWA: 18
D3S1358: 14, 18
D21S11: 28, 30
D18S51: 15
Penta E: 14, 19
Penta D: 11, 12
D8S1179: 10 ,12
FGA: 22, 25
CLS ? Cell Lines Service, 2010. | | Antigen Expression: | Blood Type B, Rh+; HLA A2, A3, Bw16, Bw47 | | Receptors Expressed: | epidermal growth factor (EGF); transforming growth factor beta (type 1 and type 2) | | Isoenzymes: | Me-2, 1; PGM3, 1-2, PGM1, 1-2, ES-D, 2; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0002 |
References:
Fogh J and Trempe G. New human tumor cell lines. In Human Tumor Cells in vitro (Fogh J. ed.). New York and London, Plenum Press, pp 115-159, 1975.
Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977.
Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977.
Vilien M et al. The specificity of the microcytotoxicity assay for cell-mediated immunity in human bladder cancer. J Urol 119: 338-42, 1978.
Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J Natl Cancer Inst 66: 1003-12, 1981.
Takeuchi Y et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J Cell Physiol 162: 315-21, 1995.
Zhang W et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J Cell Physiol 162: 348-58, 1995.
Banerjee C et al. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteoclacin gene. Proc Natl Acad Sci USA 93: 4968-73, 1996.
Morris GF et al. Transcriptional activation of the human proliferating-cell nuclear antigen promoter by p53. Proc Natl Acad Sci USA 93: 895-9, 1996.
Werner H et al. Wild-type and mutant p53 differentially regulate transcription of the insulin-like growth factor I receptor gene. Proc Natl Acad Sci USA 93: 8318-23, 1996.
Wang X et al. Variabilin, a novel RGD-contining antagonist of glycoprotein IIb-IIIa and platelet aggregation inhibitor from the gard tick Dermacentor variabilis. J Biol Chem 271: 17785-90, 1996.
Karnieli E et al. The IGF-1 receptor gene promoter is a molecular target for the Ewing's Sarcoma=Wilms' Tumor 1 fusion protein. J Biol Chem 271: 19304-9, 1996.
Schar BK et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res 57: 3841-6, 1997.
Chen WF et al. Genistein modulates the effects of parathyroid hormone in human osteoblastic SaOS-2 cells. Br J Nutr 95: 1039-47, 2006.
Wise-Draper TM et al. Apoptosis inhibition by the human DEK oncoprotein involves interference with p53 functions. Mol Cell Biol 26: 7506-19, 2006. |
| COLO-94H |