| Designation: | SW-1353 | | Depositor: | Leibovitz | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 72 years | | Gender: | female | | Tissue: | bone (humerus) | | Morphology: | fibroblast | | Celltype: | chondrosarcoma | | Growth Properties: | monolayer | | Description: | The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics. | | Culture Medium: | DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:2 is recommended | | Fluid Renewal: | 2 to 3 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | Karyotype: | hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 12
D13S317: 12, 13
D16S539: 11, 12
D5S818: 10,11
D7S820: 9, 11
THO1: 6,9
TPOX: 8,11
vWA: 16,17
D3S1358: 15,18
D21S11: 30,32.2
D18S51: 13,17
Penta E: 12,14
Penta D: 10,11
D8S1179: 10,11
FGA: 22,23
CLS ? Cell Lines Service, 2011. | | Antigen Expression: | Blood type O- | | Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 2; ES-D, 2; AK-1, 1; GLO-1, 2;
Phenotype Frequency Product: 0.00009 |
References:
Merluzzi VJ et al. Membrane-associated interleukin 1 activity on human U937 tumor cells: stimulation of PGE2 production by human chondrosarcoma cells. J Immunol 139: 166-8, 1987.
Ouyang P. An in vitro model to study mesenchymal-epithelial transformation. Biochem Biophys Res Commun 246: 771-6, 1998.
Rodriques M et al. Comparative 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin uptake in human soft tissue sarcoma cell lines. Eur J Nucl Med. 27: 1839-43, 2000.
Zenmyo M et al. p21 and parathyroid hormone-related peptide in the growth plate. Calcif Tissue Int 67: 378-81, 2000.
Rodrigues M et al. Verapamil decreases accumulation of 99Tcm-MIBI and 99Tcm-tetrofosmin in human breast cancer and soft tissue sarcoma cell lines. Nucl Med Commun. 22: 645-50, 2001.
Wu C et al. Furin-mediated processing of pro-C-type natriuretic peptide. J Biol Chem 278: 25847-52, 2003.
Kubo T et al. Inhibitory effects of a new bisphosphonate, minodronate, on proliferation and invasion of a variety of malignant bone tumor cells. J Orthop Res 24: 1138-44, 2006.
Ross S et al. High-content screening analysis of the p38 pathway: profiling of structurally related p38alpha kinase inhibitors using cell-based assays. Assay Drug Dev Technol 4: 397-409, 2006. |
| SW-1353 | | Depositor: | Leibovitz | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 72 years | | Gender: | female | | Tissue: | bone (humerus) | | Morphology: | fibroblast | | Celltype: | chondrosarcoma | | Growth Properties: | monolayer | | Description: | The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics. | | Culture Medium: | DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:2 is recommended | | Fluid Renewal: | 2 to 3 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | Karyotype: | hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 12
D13S317: 12, 13
D16S539: 11, 12
D5S818: 10,11
D7S820: 9, 11
THO1: 6,9
TPOX: 8,11
vWA: 16,17
D3S1358: 15,18
D21S11: 30,32.2
D18S51: 13,17
Penta E: 12,14
Penta D: 10,11
D8S1179: 10,11
FGA: 22,23
CLS ? Cell Lines Service, 2011. | | Antigen Expression: | Blood type O- | | Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 2; ES-D, 2; AK-1, 1; GLO-1, 2;
Phenotype Frequency Product: 0.00009 |
References:
Merluzzi VJ et al. Membrane-associated interleukin 1 activity on human U937 tumor cells: stimulation of PGE2 production by human chondrosarcoma cells. J Immunol 139: 166-8, 1987.
Ouyang P. An in vitro model to study mesenchymal-epithelial transformation. Biochem Biophys Res Commun 246: 771-6, 1998.
Rodriques M et al. Comparative 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin uptake in human soft tissue sarcoma cell lines. Eur J Nucl Med. 27: 1839-43, 2000.
Zenmyo M et al. p21 and parathyroid hormone-related peptide in the growth plate. Calcif Tissue Int 67: 378-81, 2000.
Rodrigues M et al. Verapamil decreases accumulation of 99Tcm-MIBI and 99Tcm-tetrofosmin in human breast cancer and soft tissue sarcoma cell lines. Nucl Med Commun. 22: 645-50, 2001.
Wu C et al. Furin-mediated processing of pro-C-type natriuretic peptide. J Biol Chem 278: 25847-52, 2003.
Kubo T et al. Inhibitory effects of a new bisphosphonate, minodronate, on proliferation and invasion of a variety of malignant bone tumor cells. J Orthop Res 24: 1138-44, 2006.
Ross S et al. High-content screening analysis of the p38 pathway: profiling of structurally related p38alpha kinase inhibitors using cell-based assays. Assay Drug Dev Technol 4: 397-409, 2006 |
| COLO-94H |