| Designation: | TM791 | | Depositor: | Klein | | Organism: | Homo sapiens (human) | | Gender: | Female | | Tissue: | (Bone) | | Celltype: | (Osteosarcoma) | | Growth Properties: | Monolayer | | Description: | Authentication of this cell line has revealed that the STR profile of TM791 is almost identical to HeLa and KB. Thus, the origin of this cell line remains unclear. | | Culture Medium: | DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine and 10% fetal bovine serum (MG-40, CLS order number 820400). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:3 to 1:5 is recommended | | Fluid Renewal: | 1-2 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma(PCR, DAPI), bacteria and fungi (fluoresence) were negative. | | DNA Profile (STR): | Amelogenin: X / X
CSF1PO: 9/10
D13S317: 12 / 13.3
D16S539: 9/10
D5S818: 11/12
D7S820: 8/12
THO1: 7
TPOX: 8/12
vWA: 16
D3S1358: 15/18
D21S11: 27/28
D18S51: 16
Penta E: 7/17
Penta D: 8/15
D8S1179: 13
FGA: 18/21
CLS ? Cell Lines Service, 2011.
| | Products: | GAPDH PCR product |
References:
Mengede C., Vukovic V., Glüsenkamp K.-H., J?hde E. and Rajewsky M.P. Development of acid-labile immunoconjugates for tumor-specific activation of cytotoxins. Journal of Cancer Research and Clinical Oncology, 121, Supplement 1, A52, D0I: 10.1007/BF02572172, 1995.
Lixin Liu. 216. Dissertation: Analysis of the ETAA16 Expression.. Zentrum für Kinderheilkunde der Universit?t Düsseldorf. Klinik für P?diatrische H?matologie, 2001.
K. Bienemann. Dissertation: Entwicklung einer tumorspezifischen Suizidtherapie für Osteosarkome. Klinik für P?diatrische H?matologie, Onkologie und Immunologie der Heinrich Heine Universit?t Düsseldorf, 2005 |
| COLO-94H |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 70 years |
| Gender: | male |
| Tissue: | adenocarcinoma, colorectal; colon, ascendes |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | established from the primary adenocarcinoma of the colon of a 70 year-old male, Cell Lines Service |
| Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
It is recommended to coat the tissue flasks with collagen prior to cell cultivation. |
| Split Ratio: | A ratio of 1:2 to 1:8 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,Y
CSF1PO: 11,14
D13S317: 11
D16S539: 13
D5S818: 12
D7S820: 8
THO1: 7,9.3
TPOX: 8
vWA: 15,19
D3S1358: 15,17
D21S11: 27,28
D18S51: 18
Penta E: 17
Penta D: 12,13
D8S1179: 12
FGA: 21
CLS ? Cell Lines Service, 2011. |
| Reverse Transcriptase: | negative |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR
|
| Products: | Cytokeratine 8, 18, 19 |
References:
not published |