| Designation: | U-2 OS |
| Depositor: | Lee |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 15 years |
| Gender: | female |
| Tissue: | Bone |
| Morphology: | epithelial |
| Celltype: | osteosarcoma |
| Growth Properties: | monolayer |
| Description: | J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl. Viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses. |
| Culture Medium: | DMEM: Ham's F12 medium (1:1) supplemented with 2 mM L- glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | (P11-46) hypodiploid to near tetraploid; (P111-118) modal numbers 34 to 37 and 64 to 67 with abnormalities including dicentrics,breaks, rings, and pulverizations plus acrocentric subtelocentric and minute markers |
| DNA Profile (STR): | Amelogenin: X,X
CSF1PO: 12,13
D13S317: 13,11
D16S539: 11,12
D5S818: 8,11
D7S820: 11,12
THO1: 6,9:3
TPOX: 11,12
vWA: 14,18
D3S1358: 16
D21S11: 31
D18S51: 12,14
Penta E: 10,13
Penta D: 9
D8S1179: 12,14
FGA: 20
CLS ? Cell Lines Service, 2011. |
| Antigen Expression: | Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
| Receptors Expressed: | insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF-I) |
| Isoenzymes: | PGM3, 1; PGM1, 2; ES-D, 1; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0082 |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR
|
| Products: | osteosarcoma derived growth factor (ODGF) |
References:
Ponten J et al. Two established in vitro cell lines from human mesenchymal tumours. Int J Cancer 2: 434-47, 1967.
Heldin CH et al. Chemical and biological properties of a growth factor from human-cultured osteosarcoma cells: resemblance with platelet-derived growth factor. J Cell Physiol 105: 235-46, 1980.
Graves DT et al. Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells. Science 226: 972-4, 1984.
Heldin CH et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-4, 1986.
Raile K et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J Cell Physiol 159: 531-41, 1994.
Moradpour D et al. Characterization of cell lines allowing tightly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996.
Landers JE et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res 57: 3562-8, 1997.
Campbell KJ et al. Differential regulation of NF-kappaB activation and function by topoisomerase II inhibitors. BMC Cancer 6: 101, 2006.
Gasparri F et al. Multiparametric cell cycle analysis by automated microscopy. J Biomol Screen 11: 586-98, 2006. |