| Designation: | COLO-680N | | Organism: | Homo sapiens (human) | | Ethnicity: | Black | | Age/Stage: | 57 years | | Gender: | female | | Tissue: | Esophagus | | Morphology: | epitheloid | | Celltype: | esophageal squamous-cell carcinoma | | Growth Properties: | monolayer | | Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum (MG-70, CLS order number 820700). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:4 is recommended | | Fluid Renewal: | Every 2 to 3 days | | Freeze Medium: | CM-1 (CLS order number 800125 or 800150) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | DNA Profile (STR): | Amelogenin: X,X
CSF1PO: 11,12
D13S317: 13
D16S539: 11, 12
D5S818: 11
D7S820: 10,12
THO1: 8
TPOX: 6
vWA: 17, 18
D3S1358: 15
D21S11: 27
D18S51: 19
Penta E: 7,8
Penta D: 12
D8S1179: 14, 15
FGA: 18.2
CLS ? Cell Lines Service, 2011 | | Immunology: | Cells express BMP-6 (bone morphogenetic protein) in standard cell cultivation conditions. | | Viruses: | SMRV negative, as confirmed by Real-Time PCR
|
References:
Raida M, Sarbia M, Clement JH, Adam S, Gabbert HE, Hoffken K. Expression, regulation and clinical significance of bone morphogenetic protein 6 in esophageal squamous-cell carcinoma. Int J Cancer. 83(1):38-44, 1999. |
Designation: | HRT-18 (HCT-8) |
| Depositor: | Sobrero |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 67 years; |
| Gender: | male |
| Tissue: | colon |
| Morphology: | epithelial |
| Celltype: | colorectal adenocarcinoma, ileocecal |
| Growth Properties: | monolayer |
| Description: | The HCT-8 line is identical to the HRT-18 cell line. The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
D3S1358: 17
D21S11: 29,32.2
D18S51: 11,17
Penta E: 7,14
Penta D: 9,14
D8S1179: 15
FGA: 22
CLS ? Cell Lines Service, 2011. |
| Isoenzymes: | AK-1, 1; ES-D, 1-2; GLO-1, 2; G6PD, B; PGM1, 1; PGM3, 1; Me-2, 1 |
| Reverse Transcriptase: | negative |
| Products: | carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin |
References:
Tompkins WA et al. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. Cancer Inst. 52: 1101-1110, 1974.
Nelson-Rees WA et al. Distinctive banded marker chromosomes of human tumor cell lines. Int. J. Cancer 16: 74-82, 1975.
White LJ et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996.
Lee JY, Hwang WI, Lim ST. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J Ethnopharmacol 93(2-3):409-15, 2004.
Kobel M, Budianto D, Schmitt WD, Borsi L, Siri A, Hauptmann S. Influence of various cytokines on adhesion and migration of the colorectal adenocarcinoma cell line HRT-18. Oncology 68(1):33-9, 2005. Epub 2005 Mar 2.
Jansen M, Jansen PL, Otto J, Kirtil T, Neuss S, Treutner KH, Schumpelick V. The inhibition of tumor cell adhesion on human mesothelial cells (HOMC) by phospholipids in vitro. Langenbecks Arch Surg 391(2):96-101, 2006. Epub 2006 Mar 14. |