| Designation: | HOS | | Depositor: | Riken | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 13 years | | Gender: | female | | Tissue: | bone | | Morphology: | A mixture of fibroblasts and epithelial like cells | | Celltype: | sarcoma, osteogenic | | Growth Properties: | monolayer | | Description: | HOS cells exhibit a flat morphology, low saturation density, low plating efficiency in soft agar and are sensitive to chemical and viral transformation. The cells express alkaline phosphatase under basal conditions (Clover J, 1994). | | Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:2 to 1:4 is recommended | | Fluid Renewal: | 2 to 3 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
D3S1358: 15
D21S11: 31.2
D18S51: ,14
Penta E: 7,12
Penta D: 9,10
D8S1179: 14
FGA: 24
CLS ? Cell Lines Service, 2011. | | Isoenzymes: | G6PD, B | | Viruses: | SMRV negative, as confirmed by Real-Time PCR
|
References:
McAllister RM, Gardner MB, Greene AE, Bradt C, Nichols WW, Landing BH. Cultivation in vitro of cells derived from a human osteosarcoma. Cancer 27: 397-402, 1971.
McAllister RM et al. Transformation and productive infection of human osteosarcoma cells by a feline sarcoma virus. Nat. New Biol. 230(17): 279-282, 1971.
Rhim JS, Cho HY, Huebner RJ. Non-producer human cells by murine sarcoma virus. Int. J. Cancer 15(1): 23-29, 1975.
Rhim JS et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int. J. Cancer 16: 840-849, 1975.
Rhim JS. Characterization of sarcoma-positive, leukemia-negative (S+L-) human cells induced by the feline leukemia virus pseudotype of Moloney sarcoma virus. Proc. Soc. Exp. Biol. Med.167: 597-606, 1981.
Rhim JS et al. Differential susceptibility of human cells to transformation by murine and avian sarcoma viruses. Proc. Soc. Exp. Biol. Med. 170: 350-358, 1982.
Clover J, Gowen M. Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? Bone 15(6): 585-591, 1994.
Yee A et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996.
Sunjic SB et al. The influence of 4-hydroxy-2-nonenal on proliferation, differentiation and apoptosis of human osteosarcoma cells. Biofactors 24(1): 141-148, 2005. |
| HOS |
| Depositor: | Riken |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 13 years |
| Gender: | female |
| Tissue: | bone |
| Morphology: | A mixture of fibroblasts and epithelial like cells |
| Celltype: | sarcoma, osteogenic |
| Growth Properties: | monolayer |
| Description: | HOS cells exhibit a flat morphology, low saturation density, low plating efficiency in soft agar and are sensitive to chemical and viral transformation. The cells express alkaline phosphatase under basal conditions (Clover J, 1994). |
| Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
D3S1358: 15
D21S11: 31.2
D18S51: ,14
Penta E: 7,12
Penta D: 9,10
D8S1179: 14
FGA: 24
CLS ? Cell Lines Service, 2011. |
| Isoenzymes: | G6PD, B |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR
|
References:
McAllister RM, Gardner MB, Greene AE, Bradt C, Nichols WW, Landing BH. Cultivation in vitro of cells derived from a human osteosarcoma. Cancer 27: 397-402, 1971.
McAllister RM et al. Transformation and productive infection of human osteosarcoma cells by a feline sarcoma virus. Nat. New Biol. 230(17): 279-282, 1971.
Rhim JS, Cho HY, Huebner RJ. Non-producer human cells by murine sarcoma virus. Int. J. Cancer 15(1): 23-29, 1975.
Rhim JS et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int. J. Cancer 16: 840-849, 1975.
Rhim JS. Characterization of sarcoma-positive, leukemia-negative (S+L-) human cells induced by the feline leukemia virus pseudotype of Moloney sarcoma virus. Proc. Soc. Exp. Biol. Med.167: 597-606, 1981.
Rhim JS et al. Differential susceptibility of human cells to transformation by murine and avian sarcoma viruses. Proc. Soc. Exp. Biol. Med. 170: 350-358, 1982.
Clover J, Gowen M. Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? Bone 15(6): 585-591, 1994.
Yee A et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996.
Sunjic SB et al. The influence of 4-hydroxy-2-nonenal on proliferation, differentiation and apoptosis of human osteosarcoma cells. Biofactors 24(1): 141-148, 2005. |