| Designation: | COLO-320DM |
| Organism: | Homo sapiens (human) |
| Designation: | KHOS-NP | | Depositor: | Rhim | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 13 years old | | Gender: | female | | Tissue: | Bone | | Celltype: | osteosarcoma | | Growth Properties: | monolayer | | Description: | This cell line was derived from the HOS cell line (TE-85) by transformation using Kirsten murine sarcoma virus (Ki-MSV). The cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice. The cells are useful producing MSV pseudotypes with various ecotropic and xenotropic murine leukemia viruses. Cells carry the Ki-MSV genome but do not produce infectious virus particles or viral antigens. | | Culture Medium: | Minimum essential medium Eagle (Earle's BSS) supplemented with 2 mM L-glutamine, 0.1 mM non-essential amino acids (NEAA), 1.0 mM sodium pyruvate, and 10% fetal bovine serum (MG-10, CLS order number 820100). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:2 to 1:4 is recommended | | Fluid Renewal: | 2 to 3 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | Tumorigenic: | yes, in nude mice | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
D3S1358: 15
D21S11: 31.2,32.2
D18S51: 17
Penta E: 7,12
Penta D: 9,10
D8S1179: 11,14
FGA: 24
CLS ? Cell Lines Service, 2011. | | Viruses: | SMRV negative, as confirmed by Real-Time PCR
|
References:
Rhim JS et al. Non-producer human cells induced by murine sarcoma virus. Int J Cancer 15: 23-9, 1975.
Rhim JS et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int J Cancer 16: 840-9, 1975.
Ganjavi H et al. Adenovirus-mediated p53 gene therapy in osteosarcoma cell lines: sensitization to cisplatin and doxorubicin. Cancer Gene Ther 13: 415-9, 2006.
Izbicka E et al. Molecular determinants of differential sensitivity to docetaxel and paclitaxel in human pediatric cancer models. Anticancer Res 26: 1983-8, 2006. |
| Caucasian |
| Age/Stage: | 55 years; Dukes' type C |
| Gender: | Female |
| Tissue: | colon |
| Morphology: | rounded and refractile |
| Celltype: | adenocarcinoma, colorectal |
| Culture Medium: | Ham’s F12 medium supplemented with 2mM L-glutamine and 10% fetal bovine serum (MG-60, CLS order number 820600). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 5 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X / X
CSF1PO: 11
D13S317: 11
D16S539: 11/12
D5S818: 12
D7S820: 9/12
THO1: 9
TPOX: 8/9
vWA: 15/18
D3S1358: 17
D21S11: 33.2
D18S51: 15
Penta E: 11
Penta D: 9/12
D8S1179: 13
FGA: 20
CLS ? Cell Lines Service, 2010. |
| Isoenzymes: | PGM1,1; PGM3, 2; G6PD, B; PEP-D, 1; 6PGD, A; ES-D, 1 |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR
|
| Products: | serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone |
References:
Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979.
Trainer DL et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988 |