| Designation: | Y-79 | | Depositor: | Reid | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 2.5 years old | | Gender: | female | | Tissue: | Retina | | Morphology: | multicellular clusters | | Celltype: | retinoblastoma | | Growth Properties: | suspension | | Description: | The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation. The donor had a strong maternal family history of retinoblastoma. Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor. | | Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum (MG-70, CLS order number 820700). | | Subculturing: | Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1x105 cells/ml with fresh medium and transfer into new flasks. | | Split Ratio: | A ratio of 1:2 to 1:4 is recommended | | Fluid Renewal: | Twice per week | | Freeze Medium: | CM-5 (CLS order number: 800525, 25ml, 800550, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | Karyotype: | hypertriploid, with abnormalities including dicentrics, breaks, pulverizations and minutes | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 13,14
D5S818: 11,12
D7S820: 8,9
THO1: 6,9,3
TPOX: 8
vWA: 15,18
D3S1358: 15,16
D21S11: 30,32
D18S51: 13,16
Penta E: 13,18
Penta D: 12
D8S1179: 13,16
FGA: 22
CLS ? Cell Lines Service, 2011. | | Isoenzymes: | PGM1, 1; G6PD, B; ES-D, 1; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.1373 | | Reverse Transcriptase: | negative |
References:
Reid TW et al. Characteristics of an established cell line of retinoblastoma. J Natl Cancer Inst 53: 347-360, 1974.
Gallie BL et al. Heterotransplantation of retinoblastoma into the athymic “nude” mouse. Invest Ophthalmol Vis Sci 16: 256-9, 1977.
Saari JC et al. Cellular retinol- and retinoic acid-binding proteins in transformed mammalian cells. Invest Ophthalmol Vis Sci 17: 988-92, 1978.
Wong HK et al. The human papillomavirus type 16 E7 protein complements adenovirus type 5 E1A amino-terminus-dependent trasactivation of adenovirus type 5 early genes and increases ATF and Oct-1 DNA binding activity. J Virol 70: 332-340, 1996.
Rostomily RC et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res 57: 3526-3531, 1997.
Kansara V et al. Biotin uptake and cellular translocation in human derived retinoblastoma cell line (Y-79): a role of hSMVT system. Int J Pharm 312: 43-52, 2006.
Bertolesi GE et al. Ca(v)3.1 splice variant expression during neuronal differentiation of Y-79 retinoblastoma cells. Neuroscience 141: 259-68, 2006.
Usui Y et al. Expression of costimulatiory molecules on human retinoblastoma cells Y-79: functional expression of CD40 and B7H1. Invest Ophthalmol Vis Sci 47: 4607-13, 2006. |
| COLO-94H |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 70 years |
| Gender: | male |
| Tissue: | adenocarcinoma, colorectal; colon, ascendes |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | established from the primary adenocarcinoma of the colon of a 70 year-old male, Cell Lines Service |
| Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
It is recommended to coat the tissue flasks with collagen prior to cell cultivation. |
| Split Ratio: | A ratio of 1:2 to 1:8 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,Y
CSF1PO: 11,14
D13S317: 11
D16S539: 13
D5S818: 12
D7S820: 8
THO1: 7,9.3
TPOX: 8
vWA: 15,19
D3S1358: 15,17
D21S11: 27,28
D18S51: 18
Penta E: 17
Penta D: 12,13
D8S1179: 12
FGA: 21
CLS ? Cell Lines Service, 2011. |
| Reverse Transcriptase: | negative |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR
|
| Products: | Cytokeratine 8, 18, 19 |
References:
not published |