BITTE BEACHTEN
Für den Erwerb der HNSCC Zelllinien HNO41, HNO97, HNO210, HNO223 und HNO258 müssen bestimmte Voraussetzungen erfüllt sein:
Nur gemeinnützige Institutionen k?nnen diese Zelllinien über CLS Cell Lines Service GmbH erwerben. Bitte kontaktieren Sie uns.
Ein MTA muss unterzeichnet werden.
Ohne ein unterzeichnetes Bestellformular der CLS Cell Lines Service GmbH kann kein Versand erfolgen.
| Designation: | HNO258 | | Depositor: | C. Herold-Mende | | Organism: | Homo sapiens (human) | | Ethnicity: | European | | Age/Stage: | 62 years of age | | Gender: | Male | | Tissue: | Oral Cavity | | Morphology: | Epithelial | | Celltype: | Head and neck squamous cell carcinoma (HNSCC) of the oral cavity. | | Growth Properties: | Adherent | | Culture Medium: | DMEM supplemented with L-glutamine, 4.5 g/L glucose and 10% fetal bovine serum. | | Subculturing: | Remove spent medium and rinse using PBS without calcium/magnesium. Add fresh Accutase solution and let the culture sit at 37°C for 10 minutes. Collect the cells and dispense into new flasks. Comment: A general trypsin subculture routine may be used. | | Split Ratio: | An initial ration of 1:3 is recommended. | | Fluid Renewal: | 2-3 times weekly | | Freeze Medium: | CM-1 (CLS order no. 800150, 50 ml) | | Sterility: | Tests for mycoplasma (DAPI, PCR), bacteria (PCR) and fungi were negative. | | Biosafety Level: | 1 | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8
THO1: 7
TPOX: 9,11
vWA: 16,17
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References:
Vasvari GP, Dyckhoff G, Kashfi F, Lemke B, Helmke BM, Schirrmacher V, Plinkert PK, Beckhove P, Herold-Mende C. Combination of thalidomide and cisplatin in an HNSCC model results in an enhanced anti-angiogenic activity in vitro and in vivo. Int J Cancer 121: 1697-704, 2007.
Freier K, Hofele C, Knoepfle K, Gross M, Devens F, Dyckhoff G, Plinkert P, Lichter P, Herold-Mende C. Cytogenetic characterization of head and neck squamous cell carcinoma cell lines as model systems for the functional analyses of tumor-associated genes. J Oral Pathol Med. 2010 May;39(5):382-9. doi: 10.1111/j.1600-0714.2009.00864.x. Epub 2010 Feb 8. |
| COLO-60H |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| DNA Profile (STR): | Amelogenin: X,Y
CSF1PO: 11,15
D13S317: 11
D16S539: 9,13
D5S818: 9,16
D7S820: 7.3,10
THO1: 6,9.3
TPOX: 7,10
vWA: 15,16
D3S1358: 15
D21S11: 29,33.2
D18S51: 13,15
Penta E: 11,13
Penta D: 14
D8S1179: 11
FGA: 21,24
CLS ? Cell Lines Service, 2011. |