| Designation: | Caco-2 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 72 years |
| Gender: | male |
| Tissue: | Colon |
| Morphology: | epithelial |
| Celltype: | colorectal adenocarcinoma |
| Growth Properties: | monolayer |
| Description: | This line was isolated from a primary colonic tumor. Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Caco-2 cells express retinoic acid binding protein I and retinol binding protein II and are keratin positive. |
| Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:3 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; form moderately well differentiated adenocarcinomas consistent with colonic primary (grade II) |
| Karyotype: | (P14), hypertetraploid |
| DNA Profile (STR): | Amelogenin: X , X
CSF1PO: 11
D13S317: 11, 13, 14
D16S539: 12, 13
D5S818: 12, 13
D7S820: 12
THO1: 6
TPOX: 9, 11
vWA: 16, 18
D3S1358: 14, 17
D21S11: 30
D18S51: 12
Penta E: 7
Penta D: 9
D8S1179: 12, 14
FGA: 19
CLS ? Cell Lines Service, 2011.
|
| Antigen Expression: | Blood Type O; Rh+ |
| Receptors Expressed: | heat stable enterotoxin (Sta, E. coli); epidermal growth factor (EGF) |
| Isoenzymes: | Me-2, 1; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.0187 |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
| Virus Resistance: | human immunodeficiency virus (HIV, LAV) |
References:
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