REPOTMSTAT3-GFP报告基因
品牌 | 产地 | 规格 | 价格 | 储运 |
Genomeditech | USA | 1ug | ￥800 | -20℃ |
REPOTMSTAT3报告基因是吉满生物自主研发的用于检测STAT3二聚体和STAT1/STAT3异质二聚体的转录活性水平为目的的报告基因。激活STAT3后形成STAT3二聚体和STAT1/STAT3异质二聚体，它们与SIS-inducibleelement（SIE）反应元件进行相互作用调控细胞的生长和凋亡。STAT3(signaltransducers and activators of transduction-3)报告基因与多种恶性肿瘤的发生、发展和侵袭转移关系密切。
REPOTMSTAT3报告基因主要用于监控细胞STAT3信号通路中的STAT3转录活性、肿瘤治疗药物的研发以及基因过表达和RNAi的表型分析等。
pGMSTAT3-GFP是吉满生物改造后的哺乳动物真核表达载体，在其多克隆位点插入了多个STAT3结合位点，可以高效地检测STAT3的激活水平。由于载体采用了GFP作为报告基因，更便于后续的检测。同时，对载体中预测出的其它转录因子以外的结合位点进行了适当的突变，增加了质粒的转录因子结合特异性。另外，由于质粒体积减小，使得STAT3-GFP报告基因更易于转染。
质粒图谱：
包装清单：
产品编号 | 产品名称 | 包装 |
GM-024003 | REPOTMSTAT3-GFP报告基因 | 1μg |
- | 说明书 | - |
使用说明：
pGMSTAT3-GFP可以采用常规转染方法转染哺乳动物细胞。
注意事项：
本质粒未经吉满生物允许不得用于任何商业用途，也不得移交给订货人实验室以外的任何人或单位。
为了您的健康，实验操作时请穿实验服和带一次性手套。
保存条件：
-20℃保存。
参考文献：
[1] Kang Y, Park MA, Heo SW, Park SY,Kang KW, Park PH, Kim JA.
The radio-sensitizing effect ofxanthohumol is mediated by STAT3 and EGFR suppression in doxorubicin-resistantMCF-7 human breast cancer cells.
Biochim Biophys Acta. 2012 Dec 16. pii:S0304-4165(12)00351-0. doi: 10.1016/j.bbagen.2012.12.005. [Epub ahead of print]
[2] Teng J, Wang X, Xu Z, Tang N.
HBx-dependent activation of Twistmediates STAT3 Control of epithelium-mesenchymal transition of liver cells.
J Cell Biochem. 2012 Nov 13. doi:10.1002/jcb.24450. [Epub ahead of print]
[3] Villa S, Masciocchi D, Gelain A,Meneghetti F.
The influence of the substitution patternon the molecular conformation of ureido-1,2,5-oxadiazoles, related to STAT3inhibitors: chemical behavior and structural investigation.
Chem Biodivers. 2012 Jul;9(7):1240-53.doi: 10.1002/cbdv.201100339.
[4] Han L, Yue X, Zhou X, Lan FM, You G,Zhang W, Zhang KL, Zhang CZ, Cheng JQ, Yu SZ, Pu PY, Jiang T, Kang CS.
MicroRNA-21 expression is regulated byβ-catenin/STAT3 pathway and promotes glioma cell invasion by direct targetingRECK.
CNS Neurosci Ther. 2012Jul;18(7):573-83. doi: 10.1111/j.1755-5949.2012.00344.x. Epub 2012 May 28.
[5] Kwon MJ, Ma J, Ding Y, Wang R, SunZ.
Protein kinase C-θ promotes Th17differentiation via upregulation of Stat3.
J Immunol. 2012 Jun 15;188(12):5887-97.doi: 10.4049/jimmunol.1102941. Epub 2012 May 14.
[6]Seo HS, Choi HS, Kim SR, Choi YK, WooSM, Shin I, Woo JK, Park SY, Shin YC, Ko SG.
Apigenin induces apoptosis via extrinsicpathway, inducing p53 and inhibiting STAT3 and NFκB signaling inHER2-overexpressing breast cancer cells.
Mol Cell Biochem. 2012Jul;366(1-2):319-34. doi: 10.1007/s11010-012-1310-2. Epub 2012 Apr 20.
[7] Lim EJ, Hong DY, Park JH, Joung YH,Darvin P, Kim SY, Na YM, Hwang TS, Ye SK, Moon ES, Cho BW, Do Park K, Lee HK,Park T, Yang YM.
Methylsulfonylmethane suppresses breastcancer growth by down-regulating STAT3 and STAT5b pathways.
PLoS One. 2012;7(4):e33361. doi:10.1371/journal.pone.0033361. Epub 2012 Apr 2.
[8] Hahm ER, Singh SV.
Sulforaphane inhibits constitutive andinterleukin-6-induced activation of signal transducer and activator oftranscription 3 in prostate cancer cells.
Cancer Prev Res (Phila). 2010Apr;3(4):484-94. doi: 10.1158/1940-6207.CAPR-09-0250. Epub 2010 Mar 16.
[9] Lin L, Hutzen B, Li PK, Ball S, ZuoM, DeAngelis S, Foust E, Sobo M, Friedman L, Bhasin D, Cen L, Li C, Lin J.
A novel small molecule, LLL12, inhibitsSTAT3 phosphorylation and activities and exhibits potent growth-suppressiveactivity in human cancer cells.
Neoplasia. 2010 Jan;12(1):39-50.
[10] Shin DS, Kim HN, Shin KD, Yoon YJ,Kim SJ, Han DC, Kwon BM.
Cryptotanshinone inhibits constitutivesignal transducer and activator of transcription 3 function through blockingthe dimerization in DU145 prostate cancer cells.
Cancer Res. 2009 Jan 1;69(1):193-202.doi: 10.1158/0008-5472.CAN-08-2575.
[11] Duan Z, Foster R, Bell DA, MahoneyJ, Wolak K, Vaidya A, Hampel C, Lee H, Seiden MV.
Signal transducers and activators oftranscription 3 pathway activation in drug-resistant ovarian cancer.
Clin Cancer Res. 2006 Sep1;12(17):5055-63.