REPOTMNF-KB-GFP报告基因GM…

REPO^TMNF-KB-GFP报告基因

REPO^TMNF-kB-GFP报告基因是吉满生物自主研发的用于检测NFκB转录活性水平为目的的报告基因。NF-κB基因在炎症反应、免疫应答和抑制细胞凋亡等方面有着重要的作用。

REPO^TMNF-κB-GFP报告基因主要应用于NF-κB信号通路的研究和基于NF-κB信号通路的药物研究以及癌细胞中NF-KB的调控机制的研究。

pGMNF-ΚB-GFP是吉满生物改造后的哺乳动物真核表达载体，在其多克隆位点插入了多个NF-κB结合位点，可以高效地检测NF-κB的激活水平。由于载体采用了GFP作为报告基因，更便于后续的检测。同时，对载体中预测出的其它转录因子以外的结合位点进行了适当的突变，增加了质粒的转录因子结合特异性。另外，由于质粒体积减小，使得NF-κB-GFP报告基因更易于转染。

质粒图谱：

包装清单：

使用说明：

pGMNF-κB-GFP可以采用常规转染方法转染哺乳动物细胞。

TNF-α、IL-1β和LPS等是常见的NF-κB激活剂，可作为NF-κB报告基因的阳性对照。

注意事项：

本质粒未经吉满生物允许不得用于任何商业用途，也不得移交给订货人实验室以外的任何人或单位。

为了您的健康，实验操作时请穿实验服和带一次性手套。

保存条件：

-20℃保存。

参考文献：

[1]Chang CT, Lin H, Ho TY, Li CC, Lo HY, Wu SL, Huang YF, Liang JA, Hsiang CY.

Comprehensiveassessment of host responses to ionizing radiation by nuclear factor-κBbioluminescence imaging-guided transcriptomic analysis.

PLoSOne. 2011;6(8):e23682. doi: 10.1371/journal.pone.0023682. Epub 2011 Aug 24.

[2]Xu J, Ding WF, Shao KK, Wang XD, Wang GH, Li HQ, Wang HM.

Transcriptionof promoter from the human APRIL gene regulated by Sp1 and NF-kB.

Neoplasma.2012;59(3):341-7. doi: 10.4149/neo_2012_044.

[3]Barham W, Sherrill T, Connelly L, Blackwell TS, Yull FE.

Intraductalinjection of LPS as a mouse model of mastitis: signaling visualized via anNF-κB reporter transgenic.

JVis Exp. 2012 Sep 4;(67):e4030. doi: 10.3791/4030.

[4]Salerno KM, Jing X, Diges CM, Davis BM, Albers KM.

TRAFfamily member-associated NF-kappa B activator (TANK) expression increases ininjured sensory neurons and is transcriptionally regulated by Sox11.

neuroscience.2012.11.034.

[5]Nagasaki H, Yoshimura T, Aoki N.

Real-timemonitoring of inflammation status in 3T3-L1 adipocytes possessing a secretoryGaussia luciferase gene under the control of nuclear factor-kappa B responseelement.

BiochemBiophys Res Commun. 2012 Apr 13;420(3):623-7. doi: 10.1016/j.bbrc.2012.03.049.Epub 2012 Mar 17.

[6]Wang DJ, Legesse-Miller A, Johnson EL, Coller HA.

Regulationof the let-7a-3 promoter by NF-κB.

PLoSOne. 2012;7(2):e31240. doi: 10.1371/journal.pone.0031240. Epub 2012 Feb 13.

[7]Tran TA, Nguyen AD, Chang J, Goldberg MS, Lee JK, Tansey MG.

Lipopolysaccharideand tumor necrosis factor regulate Parkin expression via nuclear factor-kappaB.

PLoSOne. 2011;6(8):e23660. doi: 10.1371/journal.pone.0023660. Epub 2011 Aug 17.

[8]Robbins D, Zhao Y.

ImagingNF-κB signaling in mice for screening anticancer drugs.

MethodsMol Biol. 2011;716:169-77. doi: 10.1007/978-1-61779-012-6_10.

[9]Dvorák Z, Vrzal R, Maurel P, Ulrichová J.

Differentialeffects of selected natural compounds with anti-inflammatory activity on theglucocorticoid receptor and NF-kappaB in HeLa cells.

ChemBiol Interact. 2006 Feb 1;159(2):117-28. Epub 2005 Nov 10

[10]Ozeki M, Tamae D, Hou DX, Wang T, Lebon T, Spitz DR, Li JJ.

Responseof cyclin B1 to ionizing radiation: regulation by NF-kappaB and mitochondrialantioxidant enzyme MnSOD.

AnticancerRes. 2004 Sep-Oct;24(5A):2657-63.