Screen Quest? FRET No Wash cAMP Assay Kit
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| Cat # | 36379 | Storage | F/D/L (see storage codes below) | | Unit Size | 1 plate | Price | $950 | | Ex (nm) | 390 | Em (nm) | 650 | | MW |
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As our kit 36376, Screen Quest? No Wash FRET cAMP Assay Kit provides another convenient assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. Compared to other commercial ELISA cAMP assay kits, this homogenous cAMP assay kit does not require a wash step or the acetylation step. The assay is based on the competition for a fixed number of anti-cAMP antibody binding sites between the fluorescent cAMP tracer and non-labeled free cAMP. Free cAMP displaces the fluorescent cAMP tracer from the HRP-cAMP/anti-cAMP antibody complex. The anti-cAMP antibody is labeled with our TR Fluor? Tb while the cAMP tracer contains our bFluor? 650. In the absence of cAMP, bFluor? 650-cAMP conjugate is bound to TR Fluor? Tb-labeled anti-cAMP antibody exclusively to have a strong FRET. However, the unlabeled free cAMP in the test sample competes for the TR Fluor? Tb-labeled anti-cAMP antibody conjugate, therefore inhibits the binding of bFluor? 650-cAMP to anti-cAMP antibody. The bFluor? 650 labeled cAMP tracer only has fluorescence lifetime of nanosecond while TR Fluor? Tb-labeled anti-cAMP antibody-bound fluorescent cAMP tracer has much longer fluorescence lifetime value due to the TR-FRET. The magnitude of FRET is proportional to the concentration of cAMP in a sample. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
