CELLtainer – 最佳传质效果
能够两用于微生物发酵和细胞培养的一次性生物反应器为什么要最佳的传质效果？在生物过程中微生物的生理状态和微环境的基础条件是混合和传质！本系统支持微生物发酵和动物细胞培养方便的过程开发和细胞扩大培养全面的检测和控制与制冷生物反应器是生物过程的关键。因此，最佳的混合和传质能够保障最佳的过程效率。同时，生物过程需要在可控的环境下运行。需要检测和控制的参数包括:温度、pH、溶氧、二氧化碳浓度、培养基组分等。CELL-tainer拥有专利的一次性生物反应器技术提供快速混合和高效传质，并且能可靠地检测培养液中的温度、pH、溶氧、转速等。CELL-tainer的混合和搅拌均优于其他类型的一次性生物反应器，包括Wave、搅拌式、乃至与传统的搅拌罐媲美。Highlights(亮点)
1、优越的混合和传质拥有专利的二维混合导致了优越的传质。 CELL-tainer的氧传递系数KLa可达400/小时，搅拌时间介于70和140秒之间。2、微生物发酵培养与细胞培养双功能
About CellutionCELLution Biotech BV is developing technologies and designing tools for application in single-use bioreactor systems. Applications are to be found both in cell cultures and microbial fermentations as well as in R&D and (GMP) Production. Technologies (IP) developed are resulting in high quality bioprocesses and aimed to improve biopharmaceutical processes: the performance, the yield, the costs. How this startedNico Oosterhuis, the founding father of CELLution Biotech, is a bioprocess engineer and scientist. His thesis at the University of Delft (SCALE-UP OF BIOREACTORS; a scale-down approach) became a very important work and is cited by many other bioprocess engineers. Since the early eighties, Nico always was involved in projects in which bioreactors played an important role. Early 2000 the wave-type single-use bioreactors were accepted for application in cell cultures, however by far these systems could not be compared with stirred tank reactors and in particular not regarding the performance. Single-use bioreactors offered reduction in cost and reduction of risk (cross contamination) and therefore became more important during the early years of this decennium. Designing a plastic copy of the stirred tank reactor is possible, however there still are many obsticals, like the agitation system, that increases the price of a single-use stirred bioreactor to an unacceptable level.
On top that, it seemed that the plastic pillow shaped bag was accepted by the market for cell cultures. However the limitations of the wave-type bioreactors remained: poor mixing and poor mass transfer and therefore only applicable with cell cultures. Even with cell cultures it was and is not possible to support high cell densitities. Microbial fermentations were almost out of the question.
But what actually is needed for an optimal performing bioprocess?
Of course with experience and expertise as built up over the last 25 years, it was clear to Nico that the physiological stage of the cell and microbial organism as well as the micro-environment are essential in a bioprocess. This means: it’s all about mixing and mass transfer.
Mass transfer is influenced by wavelength, shaking speed and shaking angle. Discovered was that also the horizontal displacement has a significant effect on the mass transfer capacity. These parameters are the basis for the design of the CELL-tainer. By keeping the mass transfer characteristics as well as the fluid flow forces constant when scaling-up, the micro-environment of the cell will be kept uniform, thus making it easy to predict large scale behavior of the cells from small scale experiments. As a result of the theoretical and practical studies, the patented two-dimensional movement came to live! With the input of highly respected biopharma industries the CELL-tainer was born and brought from prototype to what it is today: a high performance single-use bioreactor system for multiple application. >
Essential in a bioprocess are the physiological stage of the cell and microbial organism and the micro-environment. It's all about mixing and mass transfer.
The bioreactor is the core of the bioprocess, therefore mixing and mass transfer should be optimal and such that the highest possible efficiency of the process is achieved. At the same time a bioprocess needs to be executed in a controlled environment to achieve high cell density, optimal viability and high productivity. For both cell cultures and microbial fermentation parameters to be measured and controlled are Temperature, pH, pO2, pCO2, medium components a.o.
The patented technology of the single-use bioreactor CELL-tainer offers short mixing times and high mass transfer rates including reliable measurement and control of T, pH, DO and rpm in the culture medium. The mixing and mass transfer rates of CELL-tainer are equal or better than what can be achieved in wave type bioreactors, stirred single-use reactors and even in the classical CSTR.
? Single-use bioreactor for high density bacterial fermentation: evaluation of the CELL-tainer bioreactor. Results with E.coli by Sanofi Pasteur
? CELL-tainer technology supports high cell densities using serum-free suspension CHO cells
? CELL-tainer technology supports the growth of a rat-anti-mouse IgG2a producing hybridoma
? KLa / Oxygen Transfer Rate in microbial fermentation |
? A single-use bioreactor for both cell and microbial cultures: a dream becomes reality
? How Multipurpose is a single-use bioreactor, BioPharm International, March 2011
? IBS2010, Rimini: The new age in bioprocessing
? The new age in bioprocessing
? On-Line Glucose in CELL-tainer
? poster ECFG CELLtainer
? US Patent 2008/0160597 A1
? Cultivation of PER.C6 cells
? DSM_Esact2009
? ESACT_2009_PERC6_DSM
? High cell density cultures in the CELL-tainer single-use bioreactor
? MASS TRANSFER in the CELL-tainer DISPOSABLE BIOREACTOR
? Method and apparatus for cultivating cells utilizing wave motion |