ATG5 Polyclonal Antibody | ATG5 A0203.pdf |
Catalog No: A0203 | Species: Rabbit | |
Gene ID: 9474 | Isotype: IgG | |
Swiss Prot: Q9H1Y0 | Purity: Affinity purification | |
Applications: WB IHC | Cross-Reactivity: H M R | |
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| 100ug | ＄220 | Ship in 5 business days | Adding to the cart | | |
| 200ug | ＄380 | Ship in 5 business days | Adding to the cart | | |
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Alt. Names
ATG5;APG5;APG5-LIKE;APG5L;ASP;hAPG5 ;
Recommended Dilutions
WB: 1:500-1:2000
IHC: 1:50-1:200
Products data
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Western blot analysis of extracts of various cell lines, using ATG5 antibody. |
Immunohistochemistry of paraffin-embedded Colon cancer using ATG5 Antibody . | |
Immunogen
Recombinant protein of human ATG5
Storage
Store at -20oC or -80oC. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).
1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
4. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.
5. Mizushima, N. et al. (1998) Nature 395, 395-8.
6. Suzuki, K. et al. (2001) EMBO J 20, 5971-81.
7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.
8. Shintani, T. et al. (1999) EMBO J 18, 5234-41.