BAK1 Polyclonal Antibody | BAK1 A0204.pdf |
Catalog No: A0204 | Species: Rabbit | |
Gene ID: 578 | Isotype: IgG | |
Swiss Prot: Q16611 | Purity: Affinity purification | |
Applications: WB | Cross-Reactivity: H M R | |
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| 100ug | ＄220 | Ship in 5 business days | Adding to the cart | | |
| 200ug | ＄380 | Ship in 5 business days | Adding to the cart | | |
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Alt. Names
BAK1;BAK;BAK-LIKE;BCL2L7;CDN1;MGC117255;MGC3887 ;
Recommended Dilutions
WB: 1:500-1:2000
Products data
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Western blot analysis of extracts of MCF7 cell lines, using BAK1 antibody. | | |
Immunogen
N term -peptide of human BAK1
Storage
Store at -20oC or -80oC. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).
1. Gross, A. et al. (1999) Genes Dev. 13, 1899-1911.
2. Wei, M.C. et al. (2001) Science 292, 727-730.
3. Zong, W.X. et al. (2001) Genes Dev. 15, 1481-1486.
4. Degenhardt, K. et al. (2002) J. Biol. Chem. 277, 14127-14134.
5. Wei, M.C. et al. (2000) Genes Dev. 14, 2060-2071.